Pollock Steve V, Pootakham Wirulda, Shibagaki Nakako, Moseley Jeffrey L, Grossman Arthur R
Department of Plant Biology, The Carnegie Institution, 260 Panama Street, Stanford, CA 94305, USA.
Photosynth Res. 2005 Dec;86(3):475-89. doi: 10.1007/s11120-005-4048-9. Epub 2005 Nov 15.
During sulfur deprivation, the photosynthetic green alga Chlamydomonas reinhardtii develops a high-affinity sulfate uptake system and increases the expression of genes encoding proteins involved in sulfur assimilation. Although two regulatory elements, SAC1 and SAC3, have been shown to be required for normal acclimation of C. reinhardtii to sulfur deprivation, a number of other regulatory elements appear to also be involved. The molecular mechanisms by which these regulatory elements function are largely unknown. This manuscript presents our current knowledge of sulfur deprivation responses and the regulation of these responses in C. reinhardtii. In addition, we present preliminary results of a sub-saturation screen for novel sulfur acclimation mutants of C. reinhardtii. A speculative model, incorporating the activities of established regulatory elements with putative novel components of the signal transduction pathway(s) is discussed.
在硫缺乏期间，光合绿藻莱茵衣藻会形成一个高亲和力的硫酸盐摄取系统，并增加参与硫同化的蛋白质编码基因的表达。尽管已证明两个调控元件SAC1和SAC3是莱茵衣藻正常适应硫缺乏所必需的，但似乎还有许多其他调控元件也参与其中。这些调控元件发挥作用的分子机制在很大程度上尚不清楚。本手稿介绍了我们目前对莱茵衣藻硫缺乏反应及其调控的认识。此外，我们还展示了对莱茵衣藻新型硫适应突变体进行亚饱和筛选的初步结果。文中讨论了一个推测模型，该模型将已确定的调控元件的活性与信号转导途径中假定的新组分结合在一起。
