Tankiewicz A, Pawlak D, Pawlak K, Szewc D, Myśliwiec M, Buczko W
Department of Pharmacodynamics, Medical University of Białystok, Mickiewicza 2C, 15-230, Białystok, Poland.
Int Urol Nephrol. 2005;37(3):621-7. doi: 10.1007/s11255-005-0395-x.
Normocytic normochromic anaemia is a common syndrome present in patients with chronic renal insufficiency (CRI). Simultaneously in these patients the increase in L-tryptophan (TRP) degradation via kynurenine pathway is observed. On the basis of these observations we tried to examine whether one of the TRP metabolites, anthranilic acid (AA), shows interaction with membranes of erythrocytes and because of that it may contribute to anaemia development. In patients with CRI we have observed changes characteristic for normocytic normochromic anaemia, such as the decrease in erythrocyte count, haemoglobin concentration, haematocrit and the decrease in erythrocyte osmotic resistance as well as the increase in AA concentration in plasma in comparison to healthy subjects. We have also noticed the existence of a positive correlation between anthranilic acid concentration and creatinine and urea concentrations and also negative relationships between anthranilic acid concentration and haematological parameters. Moreover, incubation of healthy erythrocytes with 10 and 100 microM AA caused haemolysis curve movement to the right, which shows decrease in osmotic resistance. In conclusion, the increase in plasma AA concentration might be one of many factors, which damage erythrocyte membrane, and thereby contributes to anaemia development in patients with CRI.
正细胞正色素性贫血是慢性肾功能不全（CRI）患者中常见的综合征。同时，在这些患者中观察到通过犬尿氨酸途径的L-色氨酸（TRP）降解增加。基于这些观察结果，我们试图研究TRP代谢产物之一邻氨基苯甲酸（AA）是否与红细胞膜相互作用，以及因此它是否可能导致贫血的发生。在CRI患者中，我们观察到了正细胞正色素性贫血的特征性变化，如红细胞计数、血红蛋白浓度、血细胞比容降低，红细胞渗透抵抗力降低，与健康受试者相比，血浆中AA浓度升高。我们还注意到邻氨基苯甲酸浓度与肌酐和尿素浓度之间存在正相关，邻氨基苯甲酸浓度与血液学参数之间存在负相关。此外，用10和100微摩尔AA孵育健康红细胞会导致溶血曲线向右移动，这表明渗透抵抗力降低。总之，血浆AA浓度升高可能是损害红细胞膜并从而导致CRI患者贫血发生的众多因素之一。
