Németh J, Héjjas M, Büki B, Takács M, Schaff Z, Makó J, Szalay F
I. Belklinika, Semmelweis OTE, Budapest.
Orv Hetil. 1992 Jul 5;133 Suppl 1:24-7.
The diagnostic usefulness of anti HCV EIA test of Abbott and Ortho companies were compared. The anti HCV levels determined in the sera of 173 patients with chronic liver diseases and of 17 haemodialysed kidney patients. 109 of 190 (57%) sera were found to be negative and 81 (43%) positive determined by Abbott kit, while 127 (67%) were negative and 63 (30%) were positive by the Ortho kit. Positive results in patients with chronic liver disease were found in 66 patients by Abbott and 58 patients with Ortho Kit. Distribution of positive cases according to the diagnosis is as follows: 29 (57%) out of 51 chronic hepatitis, 17 (61%) out of 28 cirrhosis. Conflicting results were obtained in 20 cases of 190 (11%) when 17 sera were positive by Abbott and negative by ORTHO, and 3 sera were negative by Abbott, and positive by Ortho. The samples close to the cut off and with low positivity with conflicting results were checked again by the neutralization HCV EIA Abbott assay. We found the Abbott HCV EIA test more sensitive in our excellent for screening of large numbers of samples, we recommend to confirm the positive results by a neutralization type test.
比较了雅培公司和奥索公司的抗丙型肝炎病毒酶免疫分析（EIA）检测的诊断效用。测定了173例慢性肝病患者和17例接受血液透析的肾病患者血清中的抗丙型肝炎病毒水平。采用雅培试剂盒检测，190份血清中有109份（57%）为阴性，81份（43%）为阳性；而采用奥索试剂盒检测，127份（67%）为阴性，63份（30%）为阳性。雅培试剂盒检测出66例慢性肝病患者呈阳性，奥索试剂盒检测出58例呈阳性。根据诊断结果，阳性病例分布如下：51例慢性肝炎中有29例（57%），28例肝硬化中有17例（61%）。190例中有20例（11%）出现结果冲突，其中17份血清雅培检测为阳性而奥索检测为阴性，3份血清雅培检测为阴性而奥索检测为阳性。对接近临界值且阳性率低且结果冲突的样本，再次采用雅培丙型肝炎病毒中和EIA检测进行检查。我们发现雅培抗丙型肝炎病毒酶免疫分析检测在筛查大量样本时更敏感，我们建议通过中和型检测来确认阳性结果。
