Jackson Edmund S, Wayland Matt T, Fitzgerald William, Bahn Sabine
Signal Processing Laboratory, Department of Engineering, University of Cambridge, UK.
Methods. 2005 Nov;37(3):247-60. doi: 10.1016/j.ymeth.2005.09.006.
This paper will give a complete methodological approach to the processing of oligonucleotide microarray data from postmortem tissue, particularly brain matter. Attention will be drawn to each of the important stages in the process; specifically the quality control, gene expression value calculation, multiple hypothesis testing and correlation analyses. We shall initially discuss the theoretical foundations of each individual method and subsequently apply the ensemble to a sample data set to illustrate and visualise important points.
本文将给出一种完整的方法，用于处理来自死后组织（尤其是脑组织）的寡核苷酸微阵列数据。我们将关注该过程中的每个重要阶段；具体包括质量控制、基因表达值计算、多重假设检验和相关性分析。我们首先将讨论每种方法的理论基础，随后将该方法应用于一个样本数据集，以说明并直观展示要点。
