针对疑似溃疡腺型兔热病患者的土拉弗朗西斯菌诊断性聚合酶链反应的临床应用。

Clinical use of a diagnostic PCR for Francisella tularensis in patients with suspected ulceroglandular tularaemia.

作者信息

Eliasson Henrik, Sjöstedt Anders, Bäck Erik

机构信息

Department of Infectious Diseases, Orebro University, Orebro, Sweden.

出版信息

Scand J Infect Dis. 2005;37(11-12):833-7. doi: 10.1080/00365540500400951.

DOI:10.1080/00365540500400951

PMID:16308216

Abstract

A retrospective analysis to evaluate the clinical use of a diagnostic PCR for Francisella tularensis in patients with suspected ulceroglandular tularaemia was performed. 154 samples, 129 from patients with definitive tularaemia and 25 from patients where tularaemia could be ruled out, were analysed. The diagnostic PCR had a specificity of 96%, a sensitivity of 78.3%, and a Positive Predictive Value of 99%. Especially samples from encrusted lesions, even up to 4 weeks old, in patients with tularaemia, were PCR positive to a high degree when taken properly. The diagnostic PCR is useful in suspected ulceroglandular tularaemia, giving a fast and accurate diagnosis.

摘要

对疑似溃疡腺型兔热病患者使用诊断性PCR检测土拉弗朗西斯菌的临床应用进行了回顾性分析。分析了154份样本，其中129份来自确诊兔热病患者，25份来自可排除兔热病的患者。诊断性PCR的特异性为96%，敏感性为78.3%，阳性预测值为99%。特别是兔热病患者结痂病变的样本，即使长达4周，妥善采集时PCR阳性率也很高。诊断性PCR对疑似溃疡腺型兔热病有用，能快速准确地做出诊断。