Ettinger Susan N, Scase Timothy J, Oberthaler Karen T, Craft Diane M, McKnight Josephine A, Leibman Nicole F, Charney Sarah C, Bergman Philip J
Donaldson-Atwood Cancer Clinic, The Animal Medical Center, New York, NY 10021, USA.
J Am Vet Med Assoc. 2006 Apr 1;228(7):1053-62. doi: 10.2460/javma.228.7.1053.
To determine whether argyrophilic nucleolar organizing regions (AgNORs), Ki-67, and proliferating cell nuclear antigen (PCNA) scores were associated with histologic grade and survival in dogs with soft tissue sarcomas (STSs).
Retrospective study.
60 dogs with STSs.
Medical records were examined and histologic specimens were reviewed. Tissue specimens obtained from archival materials were used to prepare sections for histologic staining for AgNOR and immunohistochemical staining for Ki-67 and PCNA labeling. Follow-up monitoring was obtained by reevaluation or telephone conversations with referring veterinarians or owners.
27 (45%) STSs were grade 1, 23 (38%) were grade 2, and 10 (17%) were grade 3. The mean and median AgNOR, Ki-67, and PCNA scores were determined, and significant positive associations among AgNOR and Ki-67 scores with histologic grade and mitotic score were detected. Fifty-four dogs had adequate follow-up examinations and were included in survival analysis and evaluation of prognostic factors. Overall median survival time was > 1,306 days. Twelve of 54 (22%) dogs died of tumor-related causes. Metastatic disease developed in 8 of 54 (15%) dogs. Results of univariate analysis indicated that increased mitotic score, increased AgNOR score, increased Ki-67 score, incomplete surgical margins, noncurative intent surgery, Ki-67 score greater than the median Ki-67 score, and AgNOR score greater than the median AgNOR score were prognostic factors for decreased survival time. Results of multivariate analysis indicated that increased AgNOR score was the only prognostic factor for decreased survival time.
Results suggested that AgNORs and possibly Ki-67 should be routinely evaluated with histologic grading for STSs in dogs.
确定嗜银核仁组织区（AgNORs）、Ki-67和增殖细胞核抗原（PCNA）评分是否与软组织肉瘤（STSs）患犬的组织学分级及生存情况相关。
回顾性研究。
60只患有STSs的犬。
检查病历并复查组织学标本。从存档材料中获取的组织标本用于制备切片，进行AgNOR组织学染色以及Ki-67和PCNA标记的免疫组织化学染色。通过与转诊兽医或犬主重新评估或电话沟通进行随访监测。
27只（45%）STSs为1级，23只（38%）为2级，10只（17%）为3级。确定了AgNOR、Ki-67和PCNA评分的均值和中位数，并检测到AgNOR和Ki-67评分与组织学分级及有丝分裂评分之间存在显著正相关。54只犬进行了充分的随访检查，并纳入生存分析和预后因素评估。总体中位生存时间>1306天。54只犬中有12只（22%）死于肿瘤相关原因。54只犬中有8只（15%）发生转移。单因素分析结果表明，有丝分裂评分增加、AgNOR评分增加、Ki-67评分增加、手术切缘不完整、非根治性手术、Ki-67评分高于中位数Ki-67评分以及AgNOR评分高于中位数AgNOR评分是生存时间缩短的预后因素。多因素分析结果表明，AgNOR评分增加是生存时间缩短的唯一预后因素。
结果表明，对于犬STSs，应常规将AgNORs以及可能的Ki-67与组织学分级一起进行评估。
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