Cordeiro R A, Brilhante R S N, Rocha M F G, Rabenhorsch S H B, Moreira J L B, Grangeiro T B, Sidrim J J C
Medical Mycology Specialized Center, School of Medicine, Federal University of Ceará, Fortaleza-CE, Brazil.
Clin Exp Dermatol. 2006 Jan;31(1):122-4. doi: 10.1111/j.1365-2230.2005.01976.x.
Chronic cutaneous dermatophytoses caused by Trichophyton rubrum are common in immunocompromised patients. In immunocompetent indivuals, the disease is more often associated with onychomycosis and tinea pedis. The aim of this study was to perform antifungal susceptibility tests and genetic analysis of sequential isolates of T. rubrum from an immunocompetent patient with chronic dermatophytosis. Antifungal susceptibility tests against griseofulvin, ketoconazole, itraconazole and fluconazole were performed with sequential isolates of T. rubrum. Genetic relationship among the isolates was analysed by the random amplification of polymorphic DNA (RAPD) method. The results revealed that treatment failure was not related to the development of drug resistance, as all of the sequential T. rubrum isolates were sensitive to antifungals tested in vitro. The RAPD data demonstrated that this disease was caused by identical isolates, with no genetic differences among them, representing a single T. rubrum strain. Treatment failure and chronicity of infection do not seem to be related to antifungal resistance.
由红色毛癣菌引起的慢性皮肤癣菌病在免疫功能低下患者中很常见。在免疫功能正常个体中，该疾病更常与甲癣和足癣相关。本研究的目的是对一名患有慢性皮肤癣菌病的免疫功能正常患者的红色毛癣菌连续分离株进行抗真菌药敏试验和基因分析。用红色毛癣菌的连续分离株对灰黄霉素、酮康唑、伊曲康唑和氟康唑进行了抗真菌药敏试验。通过随机扩增多态性DNA（RAPD）方法分析了分离株之间的遗传关系。结果显示，治疗失败与耐药性的产生无关，因为所有红色毛癣菌连续分离株在体外对所测试的抗真菌药物均敏感。RAPD数据表明，该疾病由相同的分离株引起，它们之间没有遗传差异，代表单一的红色毛癣菌菌株。治疗失败和感染的慢性化似乎与抗真菌耐药性无关。
