Barros Maria Elisabete da Silva, Hamdan Júnia Soares
Dept. of Microbiology, Institute of Biological Sciences, Federal University of Minas Gerais, Av. Antonio Carlos 6627, PO Box 486, CEP: 31.270-901, Belo Horizonte, Minas Gerais, Brazil.
Can J Microbiol. 2005 Nov;51(11):983-7. doi: 10.1139/w05-100.
Onychomycosis is a common adult human mycosis, and dermatophytes of the Trichophyton genera are the most frequently isolated microorganism. Globally, from 3% to 10% of the human population is attacked by ony cho mycosis, and many cases involve toenails. The aim of this work was to determine the minimal inhibitory concentrations (MICs) of antifungal drugs (fluconazole, ketoconazole, itraconazole, terbinafine, and griseofulvin) often used for the treatment of ungueal dermatophytosis caused by Trichophyton mentagrophytes. The MICs were determined by the broth medium macrodilution method. The results showed that activities of terbinafine and itraconazole were significantly higher (MIC <0.007-0.015 microg.mL -1 and MIC = 0.062-1.000 microg.mL -1, respectively). All isolates had reduced susceptibility to fluconazole (MIC = 16 to >64 microg.mL -1). The MICs of ketoconazole and griseofulvin varied among strains, ranging from 0.125 to 2.000 microg.mL -1 for ketoconazole and from 0.25 to 2.00 microg.mL -1 for griseofulvin. These MICs were higher than those of other studies cited, possibly because of differences in culture medium used in the other studies.
甲癣是一种常见的成人真菌病，毛癣菌属皮肤癣菌是最常分离出的微生物。在全球范围内，3%至10%的人口受到甲癣的侵袭，许多病例累及趾甲。这项工作的目的是确定常用于治疗由须癣毛癣菌引起的甲部皮肤癣菌病的抗真菌药物（氟康唑、酮康唑、伊曲康唑、特比萘芬和灰黄霉素）的最低抑菌浓度（MIC）。通过肉汤培养基大稀释法测定MIC。结果表明，特比萘芬和伊曲康唑的活性显著更高（MIC分别<0.007 - 0.015μg.mL-1和MIC = 0.062 - 1.000μg.mL-1）。所有分离株对氟康唑的敏感性均降低（MIC = 16至>64μg.mL-1）。酮康唑和灰黄霉素的MIC因菌株而异，酮康唑为0.125至2.000μg.mL-1，灰黄霉素为0.25至2.00μg.mL-1。这些MIC高于其他引用研究中的MIC，可能是因为其他研究中使用的培养基不同。
