Deng Chunhui, Yao Ning, Wang Ben, Zhang Xiangmin
Department of Chemistry, Fudan University, Shanghai 200433, China.
J Chromatogr A. 2006 Jan 20;1103(1):15-21. doi: 10.1016/j.chroma.2005.11.023. Epub 2005 Nov 23.
Paeonol is an important active component present in traditional Chinese medicines (TCMs), which was used for the treatment of many diseases such as eczema. In this work, microwave-assisted extraction (MAE) was firstly combined with headspace single-drop microextraction (HS-SDME), and applied to rapid determination of paeonol in two TCMs of Cynanchum paniculatum and Paeonia suffruticosa. In the proposed method, paeonol in TCMs was isolated by using MAE, followed by extraction and concentration by HS-SDME, and detected by gas chromatography-mass spectrometry (GC-MS). The experiment parameters of MAE and HS-SDME were discussed, and the method precision, recovery and detection limit were also studied. To further demonstrate the reliability of the quantification, both the proposed method and a standard method of steam distillation (SD) were simultaneously applied to quantitative analysis of paeonol in TCM samples from different growing areas. The experimental results show that MAE-HS-SDME is a simple and rapid method for the quantitative analysis of paeonol in TCMs, and is also a potential and alternative tool for quality monitoring for the two TCMs of C. paniculatum and P. suffruticosa.
丹皮酚是中药中的一种重要活性成分，可用于治疗多种疾病，如湿疹。在本研究中，首次将微波辅助萃取（MAE）与顶空单滴微萃取（HS-SDME）相结合，并应用于快速测定徐长卿和牡丹皮这两种中药中的丹皮酚。在所提出的方法中，通过微波辅助萃取分离中药中的丹皮酚，然后通过顶空单滴微萃取进行萃取和浓缩，并采用气相色谱-质谱联用仪（GC-MS）进行检测。讨论了微波辅助萃取和顶空单滴微萃取的实验参数，还研究了该方法的精密度、回收率和检测限。为进一步证明定量的可靠性，将所提出的方法和标准水蒸气蒸馏（SD）方法同时应用于不同产地中药样品中丹皮酚的定量分析。实验结果表明，微波辅助萃取-顶空单滴微萃取是一种用于中药中丹皮酚定量分析的简单快速方法，也是徐长卿和牡丹皮这两种中药质量监测的潜在替代工具。
