顶空单滴微萃取-气相色谱-质谱联用分析中药细辛中的挥发性成分

National Institutes for Food and Drug Control, No. 2, Tiantan Xili, Beijing 100050, China.

J Anal Methods Chem. 2013;2013:380705. doi: 10.1155/2013/380705. Epub 2013 Mar 28.

A rapid headspace single-drop microextraction gas chromatography mass spectrometry (SDME-GC-MS) for the analysis of the volatile compounds in Herba Asari was developed in this study. The extraction solvent, extraction temperature and time, sample amount, and particle size were optimized. A mixed solvent of n-tridecane and butyl acetate (1 : 1) was finally used for the extraction with sample amount of 0.750 g and 100-mesh particle size at 70°C for 15 min. Under the determined conditions, the pound samples of Herba Asari were directly applied for the analysis. The result showed that SDME-GC-MS method was a simple, effective, and inexpensive way to measure the volatile compounds in Herba Asari and could be used for the analysis of volatile compounds in Chinese medicine.

本研究建立了一种快速顶空单滴微萃取气相色谱-质谱联用（SDME-GC-MS）分析方法，用于分析细辛中的挥发性化合物。对萃取溶剂、萃取温度和时间、样品量和粒径进行了优化。最终采用正十三烷和乙酸丁酯（1:1）混合溶剂，以 0.750g 样品量和 100 目粒径，在 70°C 下萃取 15min。在确定的条件下，直接对细辛样品进行分析。结果表明，SDME-GC-MS 方法是一种简单、有效、廉价的测定细辛中挥发性化合物的方法，可用于中药中挥发性化合物的分析。