Yang Jianghua, Zhu Dandan, Ju Bowei, Jiang Xiangying, Hu Junping
Department of Pharmacy, The First Affiliated Hospital, Xinjiang Medical University Urumqi 830011, Xinjiang Uyghur Autonomous Region, China.
Food and Drug Institute, Bainguoleng Mongolia Autonomous Region Urumqi 830011, Xinjiang Uyghur Autonomous Region, China.
Am J Transl Res. 2017 Feb 15;9(2):569-579. eCollection 2017.
To investigate the contents of secoiridoid compounds (i.e. sweroside, swertiamarin and gentiopicrin) from extracts, and the potential effects of extracts against carbon tetrachloride (CCl) induced liver injury in mice. The contents of swertiamarin, gentiopicroside and sweroside from different extracts were determined with high performance liquid chromatography (HPLC). CCl was used to induce acute liver injury in mice. The serum aspartate amino transferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP), total protein (TP), total bilirubin (TB), superoxide dismutase (SOD), malondialdehyde (MDA), glutathione transferase (GSH) and catalase (CAT) were measured. HE staining was performed to investigate the pathological changes of liver. Iridoid glycoside showed the highest content in the product extracted by butanol (designated as GBA), but lower in the products extracted by ethyl acetate and water designated as GEA and GW, respectively. All extracts showed protective effects against CCl induced acute liver injury in mice, among which GBA showed the maximal protective effects. extracts induced significant decrease in the serum ALT, AST, ALP and TB compared with those in the mice with acute lung injury ( < 0.01). Obvious increase was noticed in serum TP ( < 0.01). Moreover, such effects presented in a dose-dependent manner. Compared with the control group, the MDA was significantly elevated in the model group ( < 0.01), while significant decrease was observed in the levels of SOD, GSH and CAT in model group compared with the control group ( < 0.01). Whereas, such phenomenon was completely reversed by extracts in a dose-dependent manner. showed protective effects against CCl induced acute liver injury in mice.
研究提取物中裂环烯醚萜类化合物（即獐牙菜苷、獐牙菜苦苷和龙胆苦苷）的含量，以及提取物对四氯化碳（CCl）诱导的小鼠肝损伤的潜在影响。采用高效液相色谱法（HPLC）测定不同提取物中獐牙菜苦苷、龙胆苦苷和獐牙菜苷的含量。用CCl诱导小鼠急性肝损伤。检测血清天冬氨酸氨基转移酶（AST）、丙氨酸氨基转移酶（ALT）、碱性磷酸酶（ALP）、总蛋白（TP）、总胆红素（TB）、超氧化物歧化酶（SOD）、丙二醛（MDA）、谷胱甘肽转移酶（GSH）和过氧化氢酶（CAT）。进行HE染色以研究肝脏的病理变化。裂环烯醚萜苷在正丁醇提取的产物（命名为GBA）中含量最高，但在乙酸乙酯和水提取的产物（分别命名为GEA和GW）中含量较低。所有提取物均对CCl诱导的小鼠急性肝损伤具有保护作用，其中GBA的保护作用最大。与急性肝损伤小鼠相比，提取物使血清ALT、AST、ALP和TB显著降低（P<0.01）。血清TP明显升高（P<0.01）。此外，这些作用呈剂量依赖性。与对照组相比，模型组MDA显著升高（P<0.01），而模型组SOD、GSH和CAT水平与对照组相比显著降低（P<0.01）。然而，提取物以剂量依赖性方式完全逆转了这种现象。提取物对CCl诱导的小鼠急性肝损伤具有保护作用。
