Kretz O, Barbieri L, Creppy E E, Dirheimer G
Institut de Biologie Moléculaire et Cellulaire, Centre National de la Recherche Scientifique, Strasbourg, France.
Toxicology. 1992;73(3):297-304. doi: 10.1016/0300-483x(92)90071-l.
Protein synthesis was assayed in liver and kidney of mice treated with bolesatine, a toxic glycoprotein from the mushroom Boletus satanas (Lenz) which was previously shown to be an inhibitor of protein synthesis by cell-free systems in vitro and by cultured cell-lines. Protein synthesis in vivo (Swiss mice) is inhibited in a dose-dependent manner in liver and kidney. The mechanism of action does not appear to be due to RNA-N-glycosidase activity of bolesatine or a RNAase activity of this toxin on the ribosomal RNAs. Ribosomes do not appear to be damaged by pretreatment with bolesatine as judged by a poly(U) translation system. Thus bolesatine cannot be included in the group of protein synthesis inhibitors of plant origin known as ribosome-inactivating proteins (RIPs).
用鬼笔毒素（一种来自毒红菇（Lenz）的有毒糖蛋白）处理小鼠，然后测定其肝脏和肾脏中的蛋白质合成情况。先前研究表明，该毒素在体外无细胞系统和培养细胞系中均为蛋白质合成抑制剂。体内（瑞士小鼠）肝脏和肾脏中的蛋白质合成受到剂量依赖性抑制。其作用机制似乎并非源于鬼笔毒素的RNA - N - 糖苷酶活性或该毒素对核糖体RNA的核糖核酸酶活性。根据多聚尿苷酸（poly(U)）翻译系统判断，用鬼笔毒素预处理后核糖体似乎未受损伤。因此，鬼笔毒素不能归为已知的植物源蛋白质合成抑制剂——核糖体失活蛋白（RIPs）一类。
