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Bolesatine is a toxic protein (LD50 oral 3.3 mg/kg in mice) isolated from the mushroom Boletus satanas Lenz, which inhibits protein synthesis in vitro. It induces gastroenteritis in human. 2. 14C-Bolesatine, given orally to rats (30 micrograms/kg), is distributed in the gastrointestinal, tract, kidney, liver and, to a lesser extent, in the thymus, spleen and lung. Bolesatine is eliminated in faeces and urine (80% in 24h). 3. The material excreted in urine is not proteolysed, and no protease (trypsin, chymotrypsin, pronase, proteinase K, Staphylococcus aureus (strain V8) protease and pepsin) is found to hydrolyse bolesatine in either its native or denatured form. However, thermolysin hydrolysed denatured bolesatine to a protein having a Mr of about 55 kD. 4. Bolesatine is found in all the following rat liver and kidney subcellular fractions: cytoplasm, mitochondria, ribosomes, microsomes and nuclei.

博来毒素是一种从撒旦牛肝菌（Boletus satanas Lenz）中分离出的有毒蛋白质（小鼠经口半数致死量为3.3毫克/千克），它在体外可抑制蛋白质合成。它会诱发人类肠胃炎。2. 将14C标记的博来毒素以30微克/千克的剂量经口给予大鼠后，它分布于胃肠道、肾脏、肝脏，在胸腺、脾脏和肺中的分布较少。博来毒素通过粪便和尿液排出（24小时内排出80%）。3. 尿液中排出的物质未被蛋白水解，并且未发现蛋白酶（胰蛋白酶、糜蛋白酶、链霉蛋白酶、蛋白酶K、金黄色葡萄球菌（V8菌株）蛋白酶和胃蛋白酶）能水解天然或变性形式的博来毒素。然而，嗜热菌蛋白酶可将变性的博来毒素水解为一种分子量约为55千道尔顿的蛋白质。4. 在大鼠肝脏和肾脏的所有以下亚细胞组分中均发现了博来毒素：细胞质、线粒体、核糖体、微粒体和细胞核。

Bolesatine is a toxic protein (LD50 oral 3.3 mg/kg in mice) isolated from the mushroom Boletus satanas Lenz, which inhibits protein synthesis in vitro. It induces gastroenteritis in human. 2. 14C-Bolesatine, given orally to rats (30 micrograms/kg), is distributed in the gastrointestinal, tract, kidney, liver and, to a lesser extent, in the thymus, spleen and lung. Bolesatine is eliminated in faeces and urine (80% in 24h). 3. The material excreted in urine is not proteolysed, and no protease (trypsin, chymotrypsin, pronase, proteinase K, Staphylococcus aureus (strain V8) protease and pepsin) is found to hydrolyse bolesatine in either its native or denatured form. However, thermolysin hydrolysed denatured bolesatine to a protein having a Mr of about 55 kD. 4. Bolesatine is found in all the following rat liver and kidney subcellular fractions: cytoplasm, mitochondria, ribosomes, microsomes and nuclei.

博来毒素是一种从撒旦牛肝菌（Boletus satanas Lenz）中分离出的有毒蛋白质（小鼠经口半数致死量为3.3毫克/千克），它在体外可抑制蛋白质合成。它会诱发人类肠胃炎。2. 将14C标记的博来毒素以30微克/千克的剂量经口给予大鼠后，它分布于胃肠道、肾脏、肝脏，在胸腺、脾脏和肺中的分布较少。博来毒素通过粪便和尿液排出（24小时内排出80%）。3. 尿液中排出的物质未被蛋白水解，并且未发现蛋白酶（胰蛋白酶、糜蛋白酶、链霉蛋白酶、蛋白酶K、金黄色葡萄球菌（V8菌株）蛋白酶和胃蛋白酶）能水解天然或变性形式的博来毒素。然而，嗜热菌蛋白酶可将变性的博来毒素水解为一种分子量约为55千道尔顿的蛋白质。4. 在大鼠肝脏和肾脏的所有以下亚细胞组分中均发现了博来毒素：细胞质、线粒体、核糖体、微粒体和细胞核。

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