毒红菇（Boletus satanas Lenz）来源的翻译抑制剂博来毒素的特性。氨基末端序列测定及对大鼠线粒体蛋白质合成的抑制作用。
Properties of bolesatine, a translational inhibitor from Boletus satanas Lenz. Amino-terminal sequence determination and inhibition of rat mitochondrial protein synthesis.
作者信息
Kretz O, Reinbolt J, Creppy E E, Dirheimer G
机构信息
Institut de Biologie Moléculaire et Cellulaire du Centre National de la Recherche Scientifique, Strasbourg, France.
出版信息
Toxicol Lett. 1992 Dec;64-65 Spec No:763-6. doi: 10.1016/0378-4274(92)90259-m.
Bolesatine is a toxic monomeric glycoprotein of Mr 63,000 isolated from the mushroom Boletus satanas Lenz. Its N-terminal amino acid sequence was established: NH2-Thr-Trp-Arg-Ile-Tyr-Leu-Asn-Asn-Gln-Thr-Val-Lys-Leu-Ala-Leu-Leu-Leu- Pro- Asn-Gly.... It inhibits protein synthesis in isolated rat mitochondria. After 90 min of preincubation with bolesatine, the incorporation of [14C]-leucine into mitochondrial proteins was inhibited with an IC50 of 530 nM.
波勒毒素是一种从毒红菇中分离出的毒性单体糖蛋白，分子量为63,000。其N端氨基酸序列已确定：NH2-苏氨酸-色氨酸-精氨酸-异亮氨酸-酪氨酸-亮氨酸-天冬酰胺-天冬酰胺-谷氨酰胺-苏氨酸-缬氨酸-赖氨酸-亮氨酸-丙氨酸-亮氨酸-亮氨酸-亮氨酸-脯氨酸-天冬酰胺-甘氨酸……它抑制离体大鼠线粒体中的蛋白质合成。与波勒毒素预孵育90分钟后，[14C] -亮氨酸掺入线粒体蛋白质的过程受到抑制，IC50为530 nM。
Zotero 插件