Benková M, Borosková Z, Soltýs J
Helmintologický ústav SAV, Kosice.
Vet Med (Praha). 1992 Dec;36(12):717-24.
The effects were studied of four immunomodulative substances of biological origin (purified glucan, glucan with porcine immunoglobulin and zinc, transfer factor and vitamin A) on T and B lymphocytes, along with their protective effects in pigs with experimental ascariasis. These substances activated T and B lymphocytes with different intensity. The nonspecific stimulation of these cells in the blood of pigs with the immunomodulative substances intensified the protective mechanisms; this resulted in a decreased number of migrating ascaris larvae in the lungs of pigs by 17% to 65%. Of the four immunomodulative substances studied, transfer factor and glucan-based substances with porcine immunoglobulin and zinc proved to be the most effective. Both the substances exerted a significant stimulative effect on T- and B-cell populations even in the first days after infection, reaching the maximum on days 7-9 p. i. (Figs. 1 and 2). The protective effect, assessed by the reduction of migrating ascaris larvae in the lungs of pigs, was 62% and 65% using transfer factor and glucan preparation with homologous immunoglobulin and zinc, respectively. The percentage of occurrence of T and B lymphocytes, obtained by the use of purified glucan and vitamin A, was not in correlation with their protective effect (purified glucan 17%, vitamin A 50%). The immunomodulative effects of little effective purified glucan increased when combined with porcine immunoglobulin and zinc. The results are significant from the aspect of the nonspecific stimulation of immunity in ascariasis and in some other helminthoses.
研究了四种生物来源的免疫调节物质（纯化葡聚糖、含猪免疫球蛋白和锌的葡聚糖、转移因子和维生素A）对T和B淋巴细胞的影响，以及它们对实验性蛔虫病猪的保护作用。这些物质以不同强度激活T和B淋巴细胞。用免疫调节物质对猪血液中的这些细胞进行非特异性刺激可增强保护机制；这使得猪肺中迁移的蛔虫幼虫数量减少了17%至65%。在所研究的四种免疫调节物质中，转移因子以及含猪免疫球蛋白和锌的葡聚糖类物质被证明是最有效的。即使在感染后的头几天，这两种物质对T细胞和B细胞群体也都有显著的刺激作用，在感染后第7 - 9天达到最大值（图1和图2）。通过猪肺中迁移的蛔虫幼虫数量减少来评估，使用转移因子和含同源免疫球蛋白及锌的葡聚糖制剂时，保护效果分别为62%和65%。使用纯化葡聚糖和维生素A时获得的T和B淋巴细胞出现百分比与它们的保护效果不相关（纯化葡聚糖为17%，维生素A为50%）。当与猪免疫球蛋白和锌联合使用时，效果不佳的纯化葡聚糖的免疫调节作用增强。从蛔虫病和其他一些蠕虫病中免疫的非特异性刺激方面来看，这些结果具有重要意义。
