Li Jun, Xing Jianjun, Li Defa, Wang Xu, Zhao Lidan, Lv Songqiao, Huang Deshi
National Feed Engineering Technology Research Center, China Agricultural University, Beijing.
Arch Anim Nutr. 2005 Oct;59(5):303-12. doi: 10.1080/17450390500247832.
Twenty-four barrows were used to investigate the effects of beta-glucan on immune function in weaned piglets. Pigs (8.09 +/- 0.20 kg, 28 d of age) were fed a diet without or with supplemented beta-glucan (50 mg/kg feed). All pigs were injected with ovalbumin (OVA) on day 14 to investigate their humoral immune response. On day 28, lymphocytes were isolated from all pigs to determine the effects of beta-glucan on cellular immunity of pigs in vitro. Lymphocytes from six pigs of each group were incubated with 16 microg lipopolysaccharide (LPS) per ml culture medium, the remainder with an equivalent volume of culture medium alone. Samples were collected at 0, 3, 6, 12, 18, 24, and 48 h after LPS addition for determination of interleukin-6 (IL-6), tumor necrosis factor-alpha (TNF-alpha), and interleukin-10 (IL-10). On day 31, six pigs of each group were injected with either LPS (25 microg/kg BW) or an equivalent amount of sterile saline. Blood samples were collected at 3 h after LPS injection for analysis of IL-6, TNF-alpha, and IL-10 in plasma. The results indicated that dietary beta-glucan enhanced pig antibody response to OVA only in the first week after injection. In vitro, the increases of IL-6 and TNF-alpha in culture medium were partially dampened in pigs supplemented with beta-glucan when their lymphocytes were incubated with LPS, whereas the increase of IL-10 was potentiated. In vivo, dietary beta-glucan attenuated the increase of plasma IL-6 and TNF-alpha, and enhanced the increase of plasma IL-10 when pigs were challenged with LPS. These results demonstrate that beta-glucan can improve the humoral immunity of pigs and modulate cellular immunity of pigs by mitigating the elevation of pro-inflammatory cytokines and enhancing the increase of anti-inflammatory cytokines after an immunological challenge.
选用24头仔猪来研究β-葡聚糖对断奶仔猪免疫功能的影响。猪（体重8.09±0.20千克，28日龄）被饲喂不含β-葡聚糖或添加了β-葡聚糖（50毫克/千克饲料）的日粮。在第14天给所有猪注射卵清蛋白（OVA）以研究其体液免疫反应。在第28天，从所有猪中分离淋巴细胞，以在体外确定β-葡聚糖对猪细胞免疫的影响。每组6头猪的淋巴细胞与每毫升培养基中16微克脂多糖（LPS）一起孵育，其余的仅与等量体积的培养基一起孵育。在添加LPS后的0、3、6、12、18、24和48小时收集样品，用于测定白细胞介素-6（IL-6）、肿瘤坏死因子-α（TNF-α）和白细胞介素-10（IL-10）。在第31天，每组6头猪注射LPS（25微克/千克体重）或等量的无菌生理盐水。在LPS注射后3小时收集血样，用于分析血浆中的IL-6、TNF-α和IL-10。结果表明，日粮中的β-葡聚糖仅在注射后的第一周增强了猪对OVA的抗体反应。在体外，当补充β-葡聚糖的猪的淋巴细胞与LPS孵育时，培养基中IL-6和TNF-α的增加部分受到抑制，而IL-10的增加则被增强。在体内，当猪受到LPS攻击时，日粮中的β-葡聚糖减弱了血浆IL-6和TNF-α的增加，并增强了血浆IL-10的增加。这些结果表明，β-葡聚糖可以改善猪的体液免疫，并通过减轻免疫攻击后促炎细胞因子的升高和增强抗炎细胞因子的增加来调节猪的细胞免疫。
