Venugopal G, Ram G C, Bansal M P
Immunology Section, Indian Veterinary Research Institute, Izatnagar.
Vet Immunol Immunopathol. 1992 May;32(3-4):359-64. doi: 10.1016/0165-2427(92)90057-w.
Optimal in vitro testing conditions for caprine alternative complement pathway assay were determined. Effects of the following variables were tested: heterologous erythrocytes; pH, ionic strength and Mg2+ ion concentration of the complement diluent; incubation time and temperature. Rabbit erythrocytes were the optimal target cells. The optimal buffer conditions were: pH 8.0, ionic strength 0.06 mmol NaCl and 5mmol Mg2+ ion. Optimal incubation time and temperature were 75 min and 30 degrees C, respectively.
