Determination of optimal in vitro conditions for caprine alternative complement pathway assay.
作者信息
Venugopal G, Ram G C, Bansal M P
机构信息
Immunology Section, Indian Veterinary Research Institute, Izatnagar.
出版信息
Vet Immunol Immunopathol. 1992 May;32(3-4):359-64. doi: 10.1016/0165-2427(92)90057-w.
PMID:1632071
Abstract
Optimal in vitro testing conditions for caprine alternative complement pathway assay were determined. Effects of the following variables were tested: heterologous erythrocytes; pH, ionic strength and Mg2+ ion concentration of the complement diluent; incubation time and temperature. Rabbit erythrocytes were the optimal target cells. The optimal buffer conditions were: pH 8.0, ionic strength 0.06 mmol NaCl and 5mmol Mg2+ ion. Optimal incubation time and temperature were 75 min and 30 degrees C, respectively.
摘要
相似文献
1
Determination of optimal in vitro conditions for caprine alternative complement pathway assay.
Vet Immunol Immunopathol. 1992 May;32(3-4):359-64. doi: 10.1016/0165-2427(92)90057-w.
2
Studies on the haemolytic complement of the dromedary camel (Camelus dromedarius). I. Classical pathway haemolytic activity in serum.
Vet Immunol Immunopathol. 1995 Jun;46(3-4):337-47. doi: 10.1016/0165-2427(94)05367-2.
3
Study of the optimal reaction conditions for assay of the mouse alternative complement pathway.
J Immunol Methods. 1985 Dec 27;85(2):233-43. doi: 10.1016/0022-1759(85)90134-6.
4
A study of optimal reaction conditions for an assay of the human alternative complement pathway.一项关于人类替代补体途径检测的最佳反应条件的研究。
Am J Clin Pathol. 1983 Jan;79(1):65-72. doi: 10.1093/ajcp/79.1.65.
5
Hemolytic assay for goat (caprine) and swine (porcine) complement.
Vet Immunol Immunopathol. 1980 Dec;1(4):317-28. doi: 10.1016/0165-2427(80)90011-2.
6
Feline (cat) hemolytic complement optimal testing conditions.猫科动物（猫）溶血补体最佳检测条件。
Am J Vet Res. 1981 Mar;42(3):378-81.
7
Chicken (Gallus gallus) hemolytic complement: optimal conditions for its titration.
Immunol Commun. 1975;4(4):337-51. doi: 10.3109/08820137509055784.
8
Studies on the haemolytic complement of the dromedary camel (Camelus dromedarius). II. Alternate complement pathway haemolytic activity in serum.
Vet Immunol Immunopathol. 1995 Sep;48(1-2):169-76. doi: 10.1016/0165-2427(94)05406-i.
9
Demonstration of alternative and classical complement pathway activity in colostrum from buffalo (Bubalus bubalis).水牛（Bubalus bubalis）初乳中替代和经典补体途径活性的证明。
Vet Res Commun. 2003 Sep;27(6):445-52. doi: 10.1023/a:1025785506954.
10
Characterization of red blood cells strongly coated in vitro by C3 via the alternative pathway.通过替代途径在体外被C3强烈包被的红细胞的特征描述。
Transfusion. 1980 May-Jun;20(3):256-62. doi: 10.1046/j.1537-2995.1980.20380214890.x.
引用本文的文献
1
The complement system of the goat: haemolytic assays and isolation of major proteins.山羊补体系统：溶血试验和主要蛋白的分离。
BMC Vet Res. 2012 Jun 26;8:91. doi: 10.1186/1746-6148-8-91.
Zotero 插件