组织中霉菌鉴定分子方法的验证及临床应用
Validation and clinical application of molecular methods for the identification of molds in tissue.
作者信息
Paterson P J, Seaton S, McHugh T D, McLaughlin J, Potter M, Prentice H G, Kibbler C C
机构信息
Department of Medical Microbiology, Royal Free and University College Medical School, Royal Free Hospital, London, United Kingdom.
出版信息
Clin Infect Dis. 2006 Jan 1;42(1):51-6. doi: 10.1086/498111. Epub 2005 Nov 29.
BACKGROUND
Invasive fungal infections due to less-common molds are an increasing problem, and accurate diagnosis is difficult.
METHODS
We used our previously established molecular method, which allows species identification of molds in histological tissue sections, to test sequential specimens from 56 patients with invasive fungal infections who were treated at our institution from 1982 to 2000.
RESULTS
The validity of the method was demonstrated with the establishment of a molecular diagnosis in 52 cases (93%). Confirmation of the causative organism was made in all cases in which a mold had been cultured from the tissue specimen. Less-common molds were identified in 7% of cases and appear to be an increasing problem.
CONCLUSIONS
Our previously established method has proven to be of value in determining the incidence of invasive infection caused by less-common molds. Institutions should continue to pursue diagnosis of invasive fungal infections by means of tissue culture and microbiologic analysis.
背景
由罕见霉菌引起的侵袭性真菌感染问题日益严重，准确诊断困难。
方法
我们使用先前建立的分子方法，该方法可对组织学组织切片中的霉菌进行物种鉴定，对1982年至2000年在我们机构接受治疗的56例侵袭性真菌感染患者的连续标本进行检测。
结果
该方法的有效性通过52例（93%）病例的分子诊断得以证实。在所有从组织标本中培养出霉菌的病例中均确定了致病微生物。7%的病例中鉴定出罕见霉菌，且这一问题似乎日益严重。
结论
我们先前建立的方法已证明在确定由罕见霉菌引起的侵袭性感染发生率方面具有价值。各机构应继续通过组织培养和微生物分析来诊断侵袭性真菌感染。
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