两种高分辨率分子指纹分析方法用于分析侵袭性曲霉病患者分离出的多个烟曲霉菌株的比较
Comparison of two highly discriminatory molecular fingerprinting assays for analysis of multiple Aspergillus fumigatus isolates from patients with invasive aspergillosis.
作者信息
de Valk Hanneke A, Meis Jacques F G M, de Pauw Ben E, Donnelly Peter J, Klaassen Corné H W
机构信息
Department of Medical Microbiology and Infectious Diseases, Canisius Wilhelmina Hospital, Weg door Jonkerbos 100, 6532 SZ Nijmegen, The Netherlands.
出版信息
J Clin Microbiol. 2007 May;45(5):1415-9. doi: 10.1128/JCM.02423-06. Epub 2007 Mar 21.
Abstract
Two highly discriminatory fingerprinting assays, short tandem repeat typing and amplified fragment length polymorphism (AFLP), were compared to determine the genetic relatedness between 55 isolates of Aspergillus fumigatus obtained from 15 different patients suffering from proven invasive aspergillosis. Both techniques showed that interpatient isolates belonged to different genotypes and that intrapatient isolates from deep sites were all of the same genotype. By contrast, multiple genotypes were found among isolates originating from respiratory samples. Both techniques have specific advantages and disadvantages. AFLP is more universally applicable, but short tandem repeat analysis offers better discriminatory power and should be the preferred method for standardizing typing of clinical isolates of Aspergillus fumigatus.
摘要
对两种高度具有鉴别力的指纹分析方法——短串联重复序列分型和扩增片段长度多态性分析（AFLP）进行了比较，以确定从15例确诊为侵袭性曲霉病的不同患者中分离出的55株烟曲霉之间的遗传相关性。两种技术均显示，不同患者的分离株属于不同基因型，而来自深部部位的同一患者的分离株均为相同基因型。相比之下，在源自呼吸道样本的分离株中发现了多种基因型。两种技术都有其特定的优缺点。AFLP更具普遍适用性，但短串联重复序列分析具有更好的鉴别力，应作为烟曲霉临床分离株分型标准化的首选方法。
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