Jurkiewicz Piotr, Sýkora Jan, Olzyńska Agnieszka, Humpolícková Jana, Hof Martin
J. Heyrovský Institute of Physical Chemistry, Academy of Sciences of the Czech Republic, Dolejskova 3, CZ-18223, Prague 8, Czech Republic.
J Fluoresc. 2005 Nov;15(6):883-94. doi: 10.1007/s10895-005-0013-4.
Although there exist a number of methods, such as NMR, X-ray, e.g., which explore the hydration of phospholipid bilayers, the solvent relaxation (SR) method has the advantage of simple instrumentation, easy data treatment and possibility of measuring fully hydrated samples. The main information gained from SR by the analysis of recorded "time-resolved emission spectra" (TRES) is micro-viscosity and micro-polarity of the dye microenvironment. Based on these parameters, one can draw conclusions about water structure in the bilayer. In this review, we focus on physical background of this method, on all the procedures that are needed in order to obtain relevant parameters, and on the requirements on the fluorescence dyes. Furthermore, a few recent applications (the effect of curvature, binding of antibacterial peptides and phase transition) illustrating the versatility of this method are mentioned. Moreover, limitations and potential problems are discussed.
尽管存在许多方法，如核磁共振（NMR）、X射线等，可用于探究磷脂双层的水合作用，但溶剂弛豫（SR）方法具有仪器简单、数据处理容易以及能够测量完全水合样品的优点。通过分析记录的“时间分辨发射光谱”（TRES）从SR获得的主要信息是染料微环境的微粘度和微极性。基于这些参数，可以得出关于双层中水结构的结论。在这篇综述中，我们关注该方法的物理背景、获得相关参数所需的所有程序以及对荧光染料的要求。此外，还提到了一些近期应用（曲率效应、抗菌肽的结合和相变），以说明该方法的多功能性。此外，还讨论了局限性和潜在问题。
