García María Teresa, Ventosa Antonio, Mellado Encarnación
Department of Microbiology and Parasitology, Faculty of Pharmacy, University of Sevilla, Spain.
FEMS Microbiol Ecol. 2005 Sep 1;54(1):97-109. doi: 10.1016/j.femsec.2005.03.009.
There is growing interest in the development and optimization of bioremediation processes to deal with environments with high salinity that are contaminated with aromatic compounds. To estimate the diversity of moderately halophilic bacteria that could be used in such processes, enrichments were performed based on growth with a variety of aromatic compounds including phenol as a model pollutant. A group of bacteria that were able to grow over a wide range of salt concentrations were isolated, with the majority of these assigned to the genus Halomonas using phenotypic features and 16S rRNA sequences comparison. PCR amplification with degenerate primers revealed the presence in these isolates of genes encoding ring-cleaving enzymes in the beta-ketoadipate pathway for aromatic catabolism: catechol 1,2-dioxygenase and protocatechuate 3,4-dioxygenase. Furthermore, the activity of these two enzymes was detected in the newly described species Halomonas organivorans. Together, these studies indicate that moderately halophilic bacteria have the potential to catabolize aromatic compounds in environments with high salinity.
人们对于开发和优化生物修复过程以处理被芳香化合物污染的高盐环境的兴趣与日俱增。为了评估可用于此类过程的中度嗜盐细菌的多样性，基于以包括苯酚作为模型污染物在内的多种芳香化合物生长进行了富集培养。分离出了一组能够在广泛盐浓度范围内生长的细菌，利用表型特征和16S rRNA序列比较，这些细菌中的大多数被归为嗜盐单胞菌属。用简并引物进行PCR扩增表明，这些分离株中存在编码参与芳香族化合物分解代谢的β-酮己二酸途径中环裂解酶的基因：儿茶酚1,2-双加氧酶和原儿茶酸3,4-双加氧酶。此外，在新描述的食有机嗜盐单胞菌物种中检测到了这两种酶的活性。这些研究共同表明，中度嗜盐细菌有潜力在高盐环境中分解代谢芳香化合物。
