Ullah Ghanim, Jung Peter, Cornell-Bell A H
Department of Physics and Astronomy and Quantitive Biology Institute, Ohio University, Athens, 45701, USA.
Cell Calcium. 2006 Mar;39(3):197-208. doi: 10.1016/j.ceca.2005.10.009. Epub 2005 Dec 2.
Observations in cultured mouse astrocytes suggest anti-phase synchronization of cytosolic calcium concentrations in nearest neighbor cells that are coupled through gap junctions. A mathematical model is used to investigate physiologic conditions under which diffusion of the second messenger inositol (1, 4, 5)-trisphosphate (IP(3)) through gap junctions can facilitate synchronized anti-phase Ca(2+) oscillations. Our model predicts anti-phase oscillations in both cytosolic calcium and IP(3) concentrations if (a) the gap junction permeability is within a window of values and (b) IP(3) is regenerated in the astrocytes via, e.g. phospholipase C(delta). This result sheds new light on the current dispute on the mechanism of intercellular calcium signaling. It provides indirect evidence for a partially regenerative mechanism as the model excludes anti-phase synchrony in the absence of IP(3) regeneration.
对培养的小鼠星形胶质细胞的观察表明，通过间隙连接耦合的相邻细胞中，胞质钙浓度存在反相同步现象。使用数学模型研究第二信使肌醇（1,4,5）-三磷酸（IP(3)）通过间隙连接扩散促进同步反相Ca(2+)振荡的生理条件。我们的模型预测，如果（a）间隙连接通透性处于一个值的窗口内，且（b）IP(3)在星形胶质细胞中通过例如磷脂酶C（δ）再生，则胞质钙和IP(3)浓度会出现反相振荡。这一结果为当前关于细胞间钙信号传导机制的争论提供了新的线索。它为部分再生机制提供了间接证据，因为该模型排除了在没有IP(3)再生情况下的反相同步。
