Mullen O L, Dodd M C, Minton J P
J Natl Cancer Inst. 1975 Jan;54(1):229-31. doi: 10.1093/jnci/54.1.229.
Cellular immunity directed against polyoma virus-induced antigen was observed with C3H/HeJ splenic lymphoid cells from mice sensitized by a short-term immunization schedule with syngeneic polyoma 4198 and 4198V tumor cells. Polyoma specificity of the response was shown by demonstration that splenic cells from DBA/2J animals with polyoma virus-induced tumors were cytotoxic for the C3H 4198 and 4198V cells, but not for the L-M cell, another cell line of C3H origin. The polyoma-specific response in the syngeneic system was detectable with the dye exclusion assay but not with the colony inhibition procedure. Colony inhibition with the L-M cell was observed with sensitized lymphoid cells in both allogeneic and syngeneic systems.
用同基因多瘤4198和4198V肿瘤细胞短期免疫方案致敏的C3H/HeJ小鼠脾淋巴细胞，观察到针对多瘤病毒诱导抗原的细胞免疫。通过证明来自患有多瘤病毒诱导肿瘤的DBA/2J动物的脾细胞对C3H 4198和4198V细胞具有细胞毒性，但对另一种C3H来源的细胞系L-M细胞无细胞毒性，表明了反应的多瘤特异性。同基因系统中的多瘤特异性反应可用染料排除法检测到，但用集落抑制法检测不到。在同种异体和同基因系统中，致敏淋巴细胞均观察到对L-M细胞的集落抑制。
