Brown E T, Umino Y, Loi T, Solessio E, Barlow R
Center for Vision Research, SUNY Upstate Medical University, Syracuse, NY 13210, USA.
Vis Neurosci. 2005 Sep-Oct;22(5):615-8. doi: 10.1017/S0952523805225105.
Effects of anesthesia on the blood glucose of C57/BL6J mice were evaluated under conditions commonly used for testing retinal sensitivity with electroretinographic (ERG) recordings. We evaluated the effects of four anesthetics: nembutal (50 mg/kg), pentothal (100 mg/kg), avertin (240 mg/kg), and ketamine/xylazine (100 mg/kg) using saline as control. We measured blood glucose (BG) levels from tail vein blood before and 15 and 60 min following intraperitoneal injections. Fifteen minutes postinjection, all four anesthetics and saline elevated BG with ketamine/xylazine and avertin having substantially greater effects than nembutal, pentothal, and saline. Only the effects of ketamine/xylazine and avertin persisted throughout the test period. Sixty minutes after injecting ketamine/xylazine BG remained elevated at 400 +/- 42 mg/dl, a 167% increase over preinjection levels. Sixty minutes after injecting avertin BG was 288 +/- 10 mg/dl, a 59% increase over preinjection levels. No sustained elevation in BG was detected 60 min following injection of nembutal, pentothal, or saline. Because BG can affect the amplitude of the ERG, caution should be exercised in the use of ketamine/xylazine or avertin. The choice of anesthesia may also be important in diabetes and metabolism research where changes in blood glucose could impact physiological processes.
在通常用于通过视网膜电图（ERG）记录测试视网膜敏感性的条件下，评估了麻醉对C57/BL6J小鼠血糖的影响。我们评估了四种麻醉剂的效果：戊巴比妥（50毫克/千克）、硫喷妥钠（100毫克/千克）、阿佛丁（240毫克/千克）以及氯胺酮/赛拉嗪（100毫克/千克），以生理盐水作为对照。我们在腹腔注射前以及注射后15分钟和60分钟测量了尾静脉血中的血糖（BG）水平。注射后15分钟，所有四种麻醉剂和生理盐水均使血糖升高，氯胺酮/赛拉嗪和阿佛丁的作用明显大于戊巴比妥、硫喷妥钠和生理盐水。只有氯胺酮/赛拉嗪和阿佛丁的作用在整个测试期间持续存在。注射氯胺酮/赛拉嗪60分钟后，血糖仍升高至400±42毫克/分升，比注射前水平增加了167%。注射阿佛丁60分钟后，血糖为288±10毫克/分升，比注射前水平增加了59%。注射戊巴比妥、硫喷妥钠或生理盐水60分钟后未检测到血糖持续升高。由于血糖会影响ERG的振幅，因此在使用氯胺酮/赛拉嗪或阿佛丁时应谨慎。在糖尿病和代谢研究中，麻醉剂的选择也可能很重要，因为血糖变化可能会影响生理过程。
