Lapchak P A, Hefti F
Division of Neurogerontology, University of Southern California, Los Angeles 90089.
Neuroreport. 1992 May;3(5):405-8. doi: 10.1097/00001756-199205000-00007.
Effects of chronic intraventricular administration of recombinant human brain-derived neurotrophic factor (rhBDNF) or recombinant human nerve growth factor (rhNGF) on presynaptic hippocampal cholinergic function in adult rats with partial fimbrial transections were measured. Partial fimbrial transections reduced synaptosomal high affinity choline uptake, choline acetyltransferase activity, and [3H] acetylcholine synthesis by approximately 50-75%. Chronic treatment with rhBDNF failed to attenuate these lesion-induced decreases. In contrast, chronic rhNGF treatment increased all three parameters by 50-90% compared to lesioned control values. Chronic treatment with rhBDNF or rhNGF attenuated weight gain of the animals. The findings failed to provide evidence for a prominent role of BDNF in the function of adult cholinergic neurons, however, they suggest an action on central neurons involved in the regulation of food intake.
测量了成年大鼠部分穹窿横断后，慢性脑室内注射重组人脑源性神经营养因子（rhBDNF）或重组人神经生长因子（rhNGF）对海马突触前胆碱能功能的影响。部分穹窿横断使突触体高亲和力胆碱摄取、胆碱乙酰转移酶活性和[3H]乙酰胆碱合成降低了约50-75%。rhBDNF的慢性治疗未能减轻这些损伤诱导的降低。相比之下，与损伤对照组相比，rhNGF的慢性治疗使所有这三个参数增加了50-90%。rhBDNF或rhNGF的慢性治疗减弱了动物的体重增加。这些发现未能为BDNF在成年胆碱能神经元功能中的突出作用提供证据，然而，它们提示了对参与食物摄入调节的中枢神经元的作用。
