Eller Gundula, Känel Layla, Krüger Martin
Max Planck Institute for Limnology, August-Thienemann-Strasse 2, 24306 Plön, Germany.
Appl Environ Microbiol. 2005 Dec;71(12):8925-8. doi: 10.1128/AEM.71.12.8925-8928.2005.
Dissolved methane was investigated in the water column of eutrophic Lake Plusssee and compared to temperature, oxygen, and sulfide profiles. Methane concentrations and delta-13C signatures indicated a zone of aerobic methane oxidation and additionally a zone of anaerobic methane oxidation in the anoxic water body. The latter coincided with a peak in hydrogen sulfide concentration. High cell numbers of aerobic and anaerobic methane-oxidizing microorganisms were detected by fluorescence in situ hybridization (FISH) or the more sensitive catalyst-amplified reporter deposition-FISH, respectively, in these layers.
对富营养化的普卢斯湖水柱中的溶解甲烷进行了研究，并与温度、氧气和硫化物剖面进行了比较。甲烷浓度和δ-13C特征表明在缺氧水体中有一个好氧甲烷氧化区，另外还有一个厌氧甲烷氧化区。后者与硫化氢浓度峰值相吻合。通过荧光原位杂交（FISH）或更灵敏的催化剂放大报告沉积-FISH分别在这些层中检测到了高数量的好氧和厌氧甲烷氧化微生物细胞。
