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1
Legionella confirmation using real-time PCR and SYTO9 is an alternative to current methodology.使用实时聚合酶链反应（PCR）和SYTO9进行军团菌确认是当前方法的一种替代方法。
Appl Environ Microbiol. 2005 Dec;71(12):8944-8. doi: 10.1128/AEM.71.12.8944-8948.2005.
2
Comparison of SYTO9 and SYBR Green I for real-time polymerase chain reaction and investigation of the effect of dye concentration on amplification and DNA melting curve analysis.SYTO9和SYBR Green I用于实时聚合酶链反应的比较以及染料浓度对扩增和DNA熔解曲线分析影响的研究。
Anal Biochem. 2005 May 1;340(1):24-34. doi: 10.1016/j.ab.2005.01.046.
3
Inter-laboratory validation of a rapid assay for the detection and quantification of Legionella spp. in water samples.水中军团菌属检测和定量的快速检测方法的实验室间验证。
Lett Appl Microbiol. 2010 Oct;51(4):421-7. doi: 10.1111/j.1472-765X.2010.02910.x. Epub 2010 Aug 26.
4
Design and implementation of a protocol for the detection of Legionella in clinical and environmental samples.临床和环境样本中军团菌检测方案的设计与实施
Diagn Microbiol Infect Dis. 2008 Oct;62(2):125-32. doi: 10.1016/j.diagmicrobio.2008.05.004. Epub 2008 Jul 14.
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Specific detection of viable Legionella cells by combined use of photoactivated ethidium monoazide and PCR/real-time PCR.通过联合使用光活化叠氮溴化乙锭和PCR/实时PCR对存活的嗜肺军团菌细胞进行特异性检测。
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Identification and differentiation of Legionella pneumophila and Legionella spp. with real-time PCR targeting the 16S rRNA gene and species identification by mip sequencing.利用靶向16S rRNA基因的实时PCR鉴定和区分嗜肺军团菌及其他军团菌属细菌，并通过mip测序进行菌种鉴定。
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Evaluation of different nucleic acid amplification techniques for the detection of M. pneumoniae, C. pneumoniae and Legionella spp. in respiratory specimens from patients with community-acquired pneumonia.评估不同核酸扩增技术用于检测社区获得性肺炎患者呼吸道标本中的肺炎支原体、肺炎衣原体和嗜肺军团菌。
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Can J Microbiol. 2006 Jun;52(6):584-90. doi: 10.1139/w05-156.
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Quantitative detection of E. coli, E. coli O157 and other shiga toxin producing E. coli in water samples using a culture method combined with real-time PCR.采用培养法结合实时荧光定量PCR对水样中的大肠杆菌、大肠杆菌O157及其他产志贺毒素大肠杆菌进行定量检测。
J Water Health. 2006 Dec;4(4):487-98.

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Comparison of the culture method with multiplex PCR for the confirmation of Legionella spp. and Legionella pneumophila.比较培养法和多重 PCR 检测法对军团菌属和嗜肺军团菌的确认。
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Factors Influencing Contamination of Domestic Household Showers.影响家庭淋浴器污染的因素。
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PCR method for the rapid detection and discrimination of Legionella spp. based on the amplification of pcs, pmtA, and 16S rRNA genes.基于pcs、pmtA和16S rRNA基因扩增的军团菌属快速检测与鉴别PCR方法。
J Appl Genet. 2016 May;57(2):251-61. doi: 10.1007/s13353-015-0317-2. Epub 2015 Sep 30.
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Opportunistic Pathogens Mycobacterium Avium Complex (MAC) and Legionella spp. Colonise Model Shower.机会性病原体鸟分枝杆菌复合群（MAC）和嗜肺军团菌在模拟淋浴器中定殖。
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Clin Microbiol Rev. 2015 Jan;28(1):95-133. doi: 10.1128/CMR.00029-14.
7
Detection of Legionella, L. pneumophila and Mycobacterium avium complex (MAC) along potable water distribution pipelines.沿饮用水分配管道检测军团菌、嗜肺军团菌和鸟分枝杆菌复合群（MAC）。
Int J Environ Res Public Health. 2014 Jul 18;11(7):7393-405. doi: 10.3390/ijerph110707393.
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High resolution melting analysis: rapid and precise characterisation of recombinant influenza A genomes.高分辨率熔解分析：重组甲型流感病毒基因组的快速精确表征
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Hartmannella vermiformis inhibition of Legionella pneumophila cultivability.弯曲哈氏菌抑制嗜肺军团菌的可培养性。
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Detection of Mycosphaerella graminicola in wheat leaves by a microsatellite dinucleotide specific-primer.利用微卫星二核苷酸特异性引物检测小麦叶片中的小麦壳针孢菌。
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本文引用的文献

1
Comparison of SYTO9 and SYBR Green I for real-time polymerase chain reaction and investigation of the effect of dye concentration on amplification and DNA melting curve analysis.SYTO9和SYBR Green I用于实时聚合酶链反应的比较以及染料浓度对扩增和DNA熔解曲线分析影响的研究。
Anal Biochem. 2005 May 1;340(1):24-34. doi: 10.1016/j.ab.2005.01.046.
2
Demonstration of preferential binding of SYBR Green I to specific DNA fragments in real-time multiplex PCR.实时多重PCR中SYBR Green I与特定DNA片段的优先结合的证明。
Nucleic Acids Res. 2003 Nov 15;31(22):e136. doi: 10.1093/nar/gng135.
3
A case of nosocomial Legionella pneumophila pneumonia.一例医院获得性嗜肺军团菌肺炎病例。
Jpn J Infect Dis. 2003 Jun;56(3):101-2.
4
Identification methods for Legionella from environmental samples.环境样本中军团菌的鉴定方法。
Water Res. 2003 Mar;37(6):1362-70. doi: 10.1016/S0043-1354(02)00220-8.
5
Legionella pneumophila: an aquatic microbe goes astray.嗜肺军团菌：一种误入歧途的水生微生物。
FEMS Microbiol Rev. 2002 Jun;26(2):149-62. doi: 10.1111/j.1574-6976.2002.tb00607.x.
6
Distribution of Legionella longbeachae and other legionellae in Japanese potting soils.嗜肺军团菌长滩亚种及其他军团菌在日本盆栽土壤中的分布情况。
J Infect Chemother. 2001 Dec;7(4):224-7. doi: 10.1007/s101560170017.
7
Detection of legionellae in hospital water samples by quantitative real-time LightCycler PCR.采用定量实时荧光定量PCR技术检测医院水样中的军团菌。
Appl Environ Microbiol. 2001 Sep;67(9):3985-93. doi: 10.1128/AEM.67.9.3985-3993.2001.
8
Legionella: from environmental habitats to disease pathology, detection and control.军团菌：从环境栖息地到疾病病理学、检测与控制
Environ Microbiol. 1999 Aug;1(4):283-93. doi: 10.1046/j.1462-2920.1999.00046.x.
9
Detection of Legionella species in respiratory specimens using PCR with sequencing confirmation.使用聚合酶链反应（PCR）并经测序确认在呼吸道标本中检测军团菌属菌种。
J Clin Microbiol. 2000 May;38(5):1709-12. doi: 10.1128/JCM.38.5.1709-1712.2000.
10
A colony based confirmation assay for Legionella and Legionella pneumophila employing the EnviroAmp Legionella system and seroagglutination.一种基于菌落的嗜肺军团菌和军团菌确认检测方法，采用环境扩增军团菌系统和血清凝集法。
Lett Appl Microbiol. 1998 May;26(5):377-81. doi: 10.1046/j.1472-765x.1998.00354.x.

使用实时聚合酶链反应（PCR）和SYTO9进行军团菌确认是当前方法的一种替代方法。

Legionella confirmation using real-time PCR and SYTO9 is an alternative to current methodology.

作者信息

Giglio Steven, Monis Paul T, Saint Christopher P

机构信息

Australian Water Quality Centre, PMB 3, Salisbury, South Australia 5108, Australia.

出版信息

Appl Environ Microbiol. 2005 Dec;71(12):8944-8. doi: 10.1128/AEM.71.12.8944-8948.2005.

DOI:10.1128/AEM.71.12.8944-8948.2005
PMID:16332896
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1317362/
Abstract

The currently accepted culture techniques for the detection of Legionella spp. in water samples (AS/NZS 3896:1998 and ISO 11731 standard methods) are slow and laborious, requiring from 7 to 14 days for a result. We describe a fully validated rapid confirmation technique that uses real-time PCR incorporating the intercalating dye SYTO9 for the direct identification of primary cultures, significantly decreasing turnaround time and allowing faster remedial action to be taken by the industry.

摘要

目前用于检测水样中军团菌属的公认培养技术（澳大利亚和新西兰标准AS/NZS 3896:1998以及ISO 11731标准方法）耗时且费力，需要7至14天才能得出结果。我们描述了一种经过充分验证的快速确认技术，该技术使用结合了嵌入染料SYTO9的实时聚合酶链反应（PCR）直接鉴定原代培养物，显著缩短了周转时间，并使行业能够更快地采取补救措施。