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本文引用的文献

1
Aerobic anoxygenic photosynthesis genes and operons in uncultured bacteria in the Delaware River.特拉华河中未培养细菌的好氧不产氧光合作用基因及操纵子
Environ Microbiol. 2005 Dec;7(12):1896-908. doi: 10.1111/j.1462-2920.2005.00883.x.
2
Phylogenetic distribution of unusual triheme to tetraheme cytochrome subunit in the reaction center complex of purple photosynthetic bacteria.紫色光合细菌反应中心复合物中异常三血红素至四血红素细胞色素亚基的系统发育分布。
Photosynth Res. 2004;79(1):83-91. doi: 10.1023/B:PRES.0000011922.56394.92.
3
New insights into metabolic properties of marine bacteria encoding proteorhodopsins.对编码视紫红质的海洋细菌代谢特性的新见解。
PLoS Biol. 2005 Aug;3(8):e273. doi: 10.1371/journal.pbio.0030273. Epub 2005 Jul 19.
4
Roseobacter-like bacteria in red and mediterranean sea aerobic anoxygenic photosynthetic populations.红海和地中海好氧不产氧光合菌群中类玫瑰杆菌属细菌
Appl Environ Microbiol. 2005 Jan;71(1):344-53. doi: 10.1128/AEM.71.1.344-353.2005.
5
Environmental genome shotgun sequencing of the Sargasso Sea.马尾藻海的环境基因组鸟枪法测序
Science. 2004 Apr 2;304(5667):66-74. doi: 10.1126/science.1093857. Epub 2004 Mar 4.
6
ARB: a software environment for sequence data.ARB：一种用于序列数据的软件环境。
Nucleic Acids Res. 2004 Feb 25;32(4):1363-71. doi: 10.1093/nar/gkh293. Print 2004.
7
Aerobic anoxygenic photosynthesis in Roseobacter clade bacteria from diverse marine habitats.来自不同海洋栖息地的玫瑰杆菌属细菌中的有氧不产氧光合作用。
Appl Environ Microbiol. 2003 Sep;69(9):5051-9. doi: 10.1128/AEM.69.9.5051-5059.2003.
8
MrBayes 3: Bayesian phylogenetic inference under mixed models.MrBayes 3：混合模型下的贝叶斯系统发育推断。
Bioinformatics. 2003 Aug 12;19(12):1572-4. doi: 10.1093/bioinformatics/btg180.
9
Remarkable diversity of phototrophic purple bacteria in a permanently frozen Antarctic lake.在一个永久冰冻的南极湖泊中，光合紫色细菌具有显著的多样性。
Appl Environ Microbiol. 2003 Aug;69(8):4910-4. doi: 10.1128/AEM.69.8.4910-4914.2003.
10
Unsuspected diversity among marine aerobic anoxygenic phototrophs.海洋需氧不产氧光合细菌中未被发现的多样性。
Nature. 2002 Feb 7;415(6872):630-3. doi: 10.1038/415630a.

新型引物揭示了海洋需氧不产氧光合细菌之间更广泛的多样性。

Novel primers reveal wider diversity among marine aerobic anoxygenic phototrophs.

作者信息

Yutin Natalya, Suzuki Marcelino T, Béjà Oded

机构信息

Department of Biology, Technion-Israel Institute of Technology, Haifa 32000, Israel.

出版信息

Appl Environ Microbiol. 2005 Dec;71(12):8958-62. doi: 10.1128/AEM.71.12.8958-8962.2005.

DOI:10.1128/AEM.71.12.8958-8962.2005
PMID:16332899
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1317425/
Abstract

Aerobic anoxygenic phototrophic bacteria (AAnPs) were previously proposed to account for up to 11% of marine bacterioplankton and to potentially have great ecological importance in the world's oceans. Our data show that previously used primers based on the M subunit of anoxygenic photosynthetic reaction center genes (pufM) do not comprehensively identify the diversity of AAnPs in the ocean. We have designed and tested a new set of pufM-specific primers and revealed several new AAnP variants in environmental DNA samples and genomic libraries.

摘要

需氧不产氧光合细菌（AAnPs）此前被认为在海洋浮游细菌中占比高达11%，并可能在全球海洋中具有重大生态意义。我们的数据表明，先前基于不产氧光合反应中心基因（pufM）的M亚基所使用的引物并不能全面识别海洋中AAnPs的多样性。我们设计并测试了一组新的pufM特异性引物，并在环境DNA样本和基因组文库中发现了几种新的AAnP变体。