短双歧杆菌UCC 2003 hrcA基因座的遗传特征分析。
Genetic characterization of the Bifidobacterium breve UCC 2003 hrcA locus.
作者信息
Ventura Marco, Canchaya Carlos, Bernini Valentina, Del Casale Antonio, Dellaglio Franco, Neviani Erasmo, Fitzgerald Gerald F, van Sinderen Douwe
机构信息
Department of Genetics, Evolution, and Anthropology, University of Parma, Parma, Italy.
出版信息
Appl Environ Microbiol. 2005 Dec;71(12):8998-9007. doi: 10.1128/AEM.71.12.8998-9007.2005.
The bacterial heat shock response is characterized by the elevated expression of a number of chaperone complexes and transcriptional regulators, including the DnaJ and the HrcA proteins. Genome analysis of Bifidobacterium breve UCC 2003 revealed a second copy of a dnaJ gene, named dnaJ2, which is flanked by the hrcA gene in a genetic constellation that appears to be unique to the actinobacteria. Phylogenetic analysis using 53 bacterial dnaJ sequences, including both dnaJ1 and dnaJ2 sequences, suggests that these genes have followed a different evolutionary development. Furthermore, the B. breve UCC 2003 dnaJ2 gene seems to be regulated in a manner that is different from that of the previously characterized dnaJ1 gene. The dnaJ2 gene, which was shown to be part of a 2.3-kb bicistronic operon with hrcA, was induced by osmotic shock but not significantly by heat stress. This induction pattern is unlike those of other characterized dnaJ genes and may be indicative of a unique stress adaptation strategy by this commensal microorganism.
细菌热休克反应的特征是多种伴侣蛋白复合体和转录调节因子的表达升高，包括DnaJ和HrcA蛋白。短双歧杆菌UCC 2003的基因组分析揭示了一个dnaJ基因的第二个拷贝，命名为dnaJ2，它在遗传结构上侧翼是hrcA基因，这种结构似乎是放线菌所特有的。使用53个细菌dnaJ序列（包括dnaJ1和dnaJ2序列）进行的系统发育分析表明，这些基因遵循了不同的进化发展路径。此外，短双歧杆菌UCC 2003的dnaJ2基因的调控方式似乎与先前鉴定的dnaJ1基因不同。dnaJ2基因被证明是一个与hrcA组成的2.3-kb双顺反子操纵子的一部分，它由渗透压休克诱导，但受热应激诱导不明显。这种诱导模式不同于其他已鉴定的dnaJ基因，可能表明这种共生微生物具有独特的应激适应策略。
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