Carrillo-Muñoz Alfonso-Javier, Giusiano Gustavo, Cárdenes Delia, Hernández-Molina Juan-Manuel, Eraso Elena, Quindós Guillermo, Guardia Celia, del Valle Oscar, Tur-Tur Cristina, Guarro Josep
Departamento de Microbiología, ACIA, P.O. Box 10178, E-08080 Barcelona, Spain.
Int J Antimicrob Agents. 2008 Jun;31(6):540-3. doi: 10.1016/j.ijantimicag.2008.01.023. Epub 2008 Mar 25.
The in vitro antifungal activity of terbinafine against 521 clinical isolates of seven species of dermatophytes, including four onychomycosis-causative species, as well as five Scopulariopsis brevicaulis isolates was determined by a modified Clinical and Laboratory Standards Institute microdilution method. Results showed a high antifungal activity of terbinafine against all dermatophyte isolates (geometric minimal inhibitory concentration (MIC)=0.026 microg/mL; concentration inhibiting 50% of mycological growth (MIC50)=0.03 microg/mL; and concentration inhibiting 90% of mycological growth (MIC90)=0.06 microg/mL). The geometric mean MICs against onychomycosis-causative dermatophyte species was lower (0.024 microg/mL) than the global MIC. However, the in vitro activity of terbinafine against S. brevicaulis was considerably lower (geometric mean MIC=1.38 microg/mL) in comparison with dermatophytes. The antifungal activity of itraconazole was lower than that of terbinafine against these fungi. These data confirm the high in vitro antifungal activity of terbinafine against dermatophytes, under standardised conditions.
采用改良的美国临床和实验室标准协会微量稀释法，测定了特比萘芬对包括4种引起甲癣的菌种在内的7种皮肤癣菌的521株临床分离株以及5株短帚霉分离株的体外抗真菌活性。结果显示，特比萘芬对所有皮肤癣菌分离株均具有较高的抗真菌活性（几何最低抑菌浓度（MIC）=0.026μg/mL；抑制50%真菌生长的浓度（MIC50）=0.03μg/mL；抑制90%真菌生长的浓度（MIC90）=0.06μg/mL）。特比萘芬对引起甲癣的皮肤癣菌菌种的几何平均MIC较低（0.024μg/mL），低于总体MIC。然而，与皮肤癣菌相比，特比萘芬对短帚霉的体外活性相当低（几何平均MIC=1.38μg/mL）。伊曲康唑对这些真菌的抗真菌活性低于特比萘芬。这些数据证实了在标准化条件下，特比萘芬对皮肤癣菌具有较高的体外抗真菌活性。
