Issaadi Nassima, Fraize Marion, Azzouz Samira, Pétavy Anne-Françoise, Sarciron Marie-Elizabeth
Department of Parasitology and Medical Mycology EA 3741, Lyon, France.
Parasitol Res. 2006 Feb;98(3):218-26. doi: 10.1007/s00436-005-0041-7. Epub 2005 Dec 7.
The study of purified alkaline phosphatase and crude extract antigen immunogenicity from Echinococcus multilocularis was carried out on BALB/c mice. The animals were immunized, then infected with E. multilocularis metacestode. The immune response against purified alkaline phosphatase was studied. Flow cytometry analysis of the CD4+ and CD8+ lymphocyte populations showed a predominance of CD4+ populations in infected immunized mice. The specific humoral response to purified alkaline phosphatase was analyzed by enzyme-linked immunosorbent assay method. We noted a stimulation of an immunoglobulin IgG response. The isotypic profile showed a prevalence of IgG1 and IgG3 in immunized infected mice compared to IgG2a and IgG2b. In addition, analysis of the profiles of the in vitro secreted cytokines, after stimulation of the splenocytes from immunized mice, was performed. The cytokine profile was a mix of Th1/Th2 types in the infected and uninfected immunized mice. The results of this study suggest a humoral mixed Th1/Th2 response, with a high predominance of Th2 response. A similar study was conducted in mice immunized with crude total antigen. The comparison of the immune response showed an important immune response in mice immunized with purified alkaline phosphatase compared to mice immunized with the crude total antigen.
对多房棘球绦虫纯化碱性磷酸酶和粗提物抗原免疫原性的研究在BALB/c小鼠上进行。给动物免疫，然后用多房棘球绦虫的中绦期幼虫感染。研究了针对纯化碱性磷酸酶的免疫反应。对CD4+和CD8+淋巴细胞群体进行流式细胞术分析，结果显示在感染免疫的小鼠中CD4+群体占优势。通过酶联免疫吸附测定法分析了对纯化碱性磷酸酶的特异性体液反应。我们注意到免疫球蛋白IgG反应受到刺激。同型谱显示，与IgG2a和IgG2b相比，在免疫感染的小鼠中IgG1和IgG3占优势。此外，对免疫小鼠脾细胞进行刺激后，对体外分泌细胞因子的谱进行了分析。在感染和未感染的免疫小鼠中，细胞因子谱为Th1/Th2混合型。本研究结果提示存在体液混合Th1/Th2反应，其中Th2反应占高度优势。在用粗制总抗原免疫的小鼠中进行了类似研究。免疫反应比较显示，与用粗制总抗原免疫的小鼠相比，用纯化碱性磷酸酶免疫的小鼠有重要的免疫反应。
