Zhang Jun, Zhang Xinying, Cao Yang
Orthopedic Department, the First Hospital of Harbin Medical University, Harbin Heilongjiang, 150001, P. R. China
Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi. 2005 Nov;19(11):864-7.
To explore the effect of spinal neural progenitor transplantation to the cervical spinal on The brachial plexus treating brachial plexus injury with the reimplantation of the avulsed spinal roots.
avulsed injury model was made on 54 rats and they were evenly divided into 3 groups: fresh group, chronic group, control group. The spinal neural progenitor was cultured and identified. Then 10 microl (1 x 10(5)/microl) cells were labelled with BrdU and transplanted into the fresh group (15 rats survived, being model for 1 week) and the chronic group (14 rats survived, being model for 2 months). No cell was transplanted into the control group. Two months after the transplantation, the recovery of function of the injured limb was evaluated. Electrophysiologic study and immunohistochemical study of the injured limb were made.
Spinal neural progenitors were isolated from the spine and became neural sphere. The neural spheres were differentiated into neurons and astrocytes. Fourteen rats out of 15 in the fresh group were recovered, 7 rats out of 14 in the chronic group were recovered, and 5 rats out of 12 in the control group were recovered. Immunohistochemical study indicated that the transplanted progenitors in fresh group survived and differentiated into the neural cells, and the transplanted progenitors in chronic group existed and did not differentiate well.
Transplanted spinal neural progenitors can promote the recovery of the brachial plexus injury with the reimplantation of the avulsed spinal root.
探讨脊髓神经祖细胞移植至颈段脊髓对臂丛神经撕脱伤再植修复后臂丛神经损伤的影响。
将54只大鼠制成臂丛神经撕脱伤模型，并平均分为3组：新鲜组、慢性组、对照组。培养并鉴定脊髓神经祖细胞。然后将10微升（1×10⁵/微升）用溴脱氧尿苷标记的细胞移植到新鲜组（存活15只大鼠，造模1周）和慢性组（存活14只大鼠，造模2个月）。对照组不移植细胞。移植后2个月，评估伤肢功能恢复情况。对伤肢进行电生理研究和免疫组化研究。
从脊髓分离出脊髓神经祖细胞并形成神经球。神经球分化为神经元和星形胶质细胞。新鲜组15只大鼠中有14只恢复，慢性组14只大鼠中有7只恢复，对照组12只大鼠中有5只恢复。免疫组化研究表明，新鲜组移植的祖细胞存活并分化为神经细胞，慢性组移植的祖细胞存在但分化不佳。
移植脊髓神经祖细胞可促进臂丛神经撕脱伤再植修复后臂丛神经损伤的恢复。
