Wang Chunsheng, Shi Zhibin, Wang Kunzheng
Department of Orthopedics, the Second Hospital of Xi'an Jiaotong University, Xi'an Shaanxi, 710004, P. R. China.
Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi. 2005 Nov;19(11):875-8.
To study the effect of olfactory ensheathing cells (OECs) transplantation on protecting spinal cord and neurons after peripheral nerve injury.
Fifty-five SD rats were randomly divided into blank group (n = 5), experimental group (n = 25) and control group (n = 25). The right sciatic nerves of all the rats were transected. The proximal end was embedded in muscle and treated with OECs (experimental group) and DMEM (control group). No treatment was given to the blank group. The rats were sacrificed 1, 2, 3, 7, and 14 days after the transplantation, the related neurons were observed with histological and TUNEL methods.
After sciatic nerves were transected, death of neurons occurred in spinal cord and ganglion. One, 2, 3 days after treatment, the neuron survival rate in experimental group was 98.4% +/- 6.5%, 97.6% +/- 6.5%, 95.2% +/- 6.7% respectively. The neuron survival rate in control group was 97.8% +/- 6.7%, 97.4% +/- 6.4%, 94.3% +/- 6.8% 1, 2, and 3 days after treatment respectively. There was no significant difference between experimental group and control group. Seven and 14 days after treatment, the neuron survival rate in experimental group was 92.4% +/- 8.9%, 87.7% +/- 9.4% respectively. The neuron survival rate in control group was 87.4% +/- 8.6%, 83.4% +/- 8.5% 7 and 14 days after treatment respectively. There was significant difference between experimental group and control group. On 1st and 2nd day, no apoptosis was seen in spinal cord anterior horn of the rats in both experimental group and control group. On 3rd, 7th, and 14th day, the apoptosis index of spinal cord anterior horn motoneuron in experimental rats were lower (1.2+/-0.8, 1.4 +/- 0. 6, 4.1 +/- 1. 3) than that in the control group (2.1 +/- 1.1, 3.1 +/- 1.1, 6.1 +/- 1.8) (P < 0.05). One, 2, and 3 days after the operation, no ganglion neurons apoptosis was observed in all rats. On 7th day the apoptosis index of ganglion neurons in experimental group(2.10 +/- 0.32) were lower than that in control group (4.40+/-0.56) (P<0.05). On 14th day there was no significant difference in the apoptosis index of ganglion neurons between experimental group (4.30+/-1.80) and control group (6.70 +/- 2.50) (P<0.05).
Apoptosis of neurons occur after peripheral nerve injury in spinal cord and ganglion. OECs transplantation is effective in preventing apoptosis.
研究嗅鞘细胞（OECs）移植对周围神经损伤后脊髓及神经元的保护作用。
55只SD大鼠随机分为空白组（n = 5）、实验组（n = 25）和对照组（n = 25）。所有大鼠右侧坐骨神经均被切断。将近端埋入肌肉，实验组用嗅鞘细胞处理，对照组用DMEM处理。空白组不做处理。移植后1、2、3、7和14天处死大鼠，采用组织学和TUNEL方法观察相关神经元。
坐骨神经切断后，脊髓和神经节中的神经元发生死亡。处理后1、2、3天，实验组神经元存活率分别为98.4%±6.5%、97.6%±6.5%、95.2%±6.7%。对照组处理后1、2、3天神经元存活率分别为97.8%±6.7%、97.4%±6.4%、94.3%±6.8%。实验组与对照组之间无显著差异。处理后7天和14天，实验组神经元存活率分别为92.4%±8.9%、87.7%±9.4%。对照组处理后7天和14天神经元存活率分别为87.4%±8.6%、83.4%±8.5%。实验组与对照组之间有显著差异。第1天和第2天，实验组和对照组大鼠脊髓前角均未观察到凋亡。第3天、第7天和第14天，实验组大鼠脊髓前角运动神经元凋亡指数低于对照组（1.2±0.8，1.4±0.6，4.1±1.3）（2.1±1.1，3.1±1.1，6.1±1.8）（P<0.05）。术后1、2、3天，所有大鼠神经节神经元均未观察到凋亡。第7天，实验组神经节神经元凋亡指数（2.10±0.32）低于对照组（4.40±0.56）（P<0.05）。第14天，实验组（4.30±1.80）和对照组（6.70±2.50）神经节神经元凋亡指数无显著差异（P<0.05）。
周围神经损伤后脊髓和神经节中的神经元发生凋亡。嗅鞘细胞移植可有效预防凋亡。
