Dong Xinjiao
School of Science of Life & Environment, Wenzhou Normal College, China.
J Environ Biol. 2005 Jul;26(3):475-8.
Cells of Penicillium jensenii were immobilized by entrapment in natural and synthetic polymeric matrices. The decolorization of Reactive Brilliant Blue KN-R by immobilized cells has been studied. It was found that CA-immobilized cells could effectively decolorize reactive brilliant blue KN-R. Many factors affecting the decolorization process were studied, including: pH, temperature, dye concentration, shaker speed and culture time. The reusability of the immobilized cells was evaluated with repeated-batch decolorization experiments. The optimum pH, temperature, shaker speed and culture time of decolorization with CA-, CGN-, and PAA- immobilized cells are 4.0 and 30 degrees C and 150r/min and 48hr respectively, dye concentration could have some effects on decolorization. After four repeated experiments, the decolorization rate of CA-, CGN-, and PAA- immobilized cells could still remain 73.6%, 60.8%, 50.5%, respectively.
詹氏青霉菌细胞通过包埋在天然和合成聚合物基质中进行固定化。研究了固定化细胞对活性艳蓝KN-R的脱色作用。发现醋酸纤维素固定化细胞能够有效脱色活性艳蓝KN-R。研究了许多影响脱色过程的因素，包括：pH值、温度、染料浓度、摇床转速和培养时间。通过重复分批脱色实验评估了固定化细胞的可重复使用性。醋酸纤维素、羧甲基壳聚糖和聚丙烯酸固定化细胞脱色的最佳pH值、温度、摇床转速和培养时间分别为4.0、30℃、150转/分钟和48小时，染料浓度会对脱色产生一定影响。经过四次重复实验后，醋酸纤维素、羧甲基壳聚糖和聚丙烯酸固定化细胞的脱色率仍分别保持在73.6%、60.8%、50.5%。
