Université de Sfax, Ecole Nationale d'Ingénieurs de Sfax, Laboratoire de Génie Enzymatique et de Microbiologie, Route de Soukra Km 4.5, BP «1173», 3038 Sfax, Tunisia.
J Environ Manage. 2013 Nov 15;129:324-32. doi: 10.1016/j.jenvman.2013.07.026. Epub 2013 Aug 26.
In this paper we studied the ability of four Tunisian-isolated fungi (i.e. Coriolopsis gallica, Bjerkandera adusta, Trametes versicolor and Trametes trogii) immobilized into Ca-alginate beads to decolorize the metal textile dye Lanaset Grey G (LG). The effect of different operational conditions, such as initial dye concentration, temperature, pH, beads/medium ratio and agitation, on dye decolorization by the immobilized fungi was investigated. Maximal decolorization percentages of 88.7%, 89.3%, 82.1% and 81.3% for C. gallica, B. adusta, T. versicolor and T. trogii were attained, respectively, when operating at an initial LG concentration of 150 mg/L, pH values of 5.0-6.0, temperatures of 40-45 °C and a beads/medium ratio of 20% (w/v) in static conditions after 72 h of incubation. Subsequently, the re-usability of the immobilized fungi was evaluated. After three decolorization cycles, the decolorization percentage of free cell cultures dropped to values below 36%, while decolorization percentages of about 75%, 70%, 60% and 68% were obtained by the immobilized cultures of C. gallica, B. adusta, T. versicolor and T. trogii, respectively.
在本文中，我们研究了 4 株分离自突尼斯的真菌（即卷枝毛霉、鲍氏卧孔菌、栓菌和粗毛纤孔菌）固定在 Ca-海藻酸钠珠中的能力，以对金属纺织染料 Lanaset Grey G（LG）进行脱色。考察了不同操作条件，如初始染料浓度、温度、pH 值、珠粒/介质比和搅拌对固定化真菌脱色的影响。当在初始 LG 浓度为 150mg/L、pH 值为 5.0-6.0、温度为 40-45°C 和在静态条件下以 20%（w/v）的珠粒/介质比下孵育 72 小时时，C. gallica、B. adusta、T. versicolor 和 T. trogii 的最大脱色率分别达到 88.7%、89.3%、82.1%和 81.3%。随后，评估了固定化真菌的可重复使用性。经过三次脱色循环后，游离细胞培养物的脱色率降至 36%以下，而 C. gallica、B. adusta、T. versicolor 和 T. trogii 的固定化培养物的脱色率分别约为 75%、70%、60%和 68%。
