基本的RNA聚合酶II转录机制。
The basic RNA polymerase II transcriptional machinery.
作者信息
Weinmann R
机构信息
Wistar Institute of Anatomy and Biology, Philadelphia, PA 19104.
出版信息
Gene Expr. 1992;2(2):81-91.
Abstract
All genes encoding proteins in eukaryotes are transcribed by RNA polymerase II. The first step in analyzing transcriptional regulation requires understanding the general mechanisms of RNA polymerase II-specific gene transcription. The basal promoter, a template containing a TATA box devoid of upstream regulatory sequences, has been used to identify and characterize the factors which, together with RNA polymerase II, govern transcription in mammalian systems: TFIIA, TFIIB, TFIID, TFIIE, TFIIF, TFIIG, TFIIH, and TFIIJ. Interactions between regulatory transcription factors and basal elements of the transcriptional machinery affect the transcriptional rate in a positive or negative fashion. As these multiple proteins are purified, and their coding sequences are isolated, we come closer to reproducing these processes in vitro with pure components, and thus to elucidating the complex interactions among them.
摘要
真核生物中所有编码蛋白质的基因均由RNA聚合酶II转录。分析转录调控的第一步需要了解RNA聚合酶II特异性基因转录的一般机制。基础启动子是一个不含上游调控序列的含有TATA框的模板，已被用于鉴定和表征与RNA聚合酶II一起在哺乳动物系统中调控转录的因子：TFIIA、TFIIB、TFIID、TFIIE、TFIIF、TFIIG、TFIIH和TFIIJ。调控转录因子与转录机制的基础元件之间的相互作用以正向或负向方式影响转录速率。随着这些多种蛋白质的纯化以及它们编码序列的分离，我们越来越接近于在体外使用纯组分重现这些过程，从而阐明它们之间复杂的相互作用。
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