Flores O, Lu H, Reinberg D
Department of Biochemistry, Robert Wood Johnson Medical School, University of Medicine and Dentistry of New Jersey, Piscataway 08854-5635.
J Biol Chem. 1992 Feb 5;267(4):2786-93.
Two new factors required for transcription of class II genes have been identified. These factors, TFIIH and TFIIJ, were required together with the previously described general factors (TFIIA, TFIIB, TFIID, TFIIE, and TFIIF) and RNA polymerase II for transcription of different class II genes. TFIIH was extensively purified, and the activity appeared to coelute with polypeptides of 33 and 95 kDa. The role of TFIIH and TFIIJ in preinitiation complex assembly was analyzed using mobility shift assays. It was found that TFIIH and TFIIJ association with the preinitiation complex was ordered and required the previous assembly of a preinitiation complex intermediate containing factors IID, IIB, IIF, IIE, and RNA polymerase II. A model for the ordered assembly of the general factors and RNA polymerase II is presented.
已鉴定出II类基因转录所需的两个新因子。这些因子，即TFIIH和TFIIJ，与先前描述的通用因子（TFIIA、TFIIB、TFIID、TFIIE和TFIIF）以及RNA聚合酶II一起，是不同II类基因转录所必需的。TFIIH被大量纯化，其活性似乎与33 kDa和95 kDa的多肽共洗脱。使用迁移率变动分析来分析TFIIH和TFIIJ在起始前复合物组装中的作用。发现TFIIH和TFIIJ与起始前复合物的结合是有序的，并且需要先前组装一个包含因子IID、IIB、IIF、IIE和RNA聚合酶II的起始前复合物中间体。本文提出了一个通用因子和RNA聚合酶II有序组装的模型。
