基本RNA聚合酶II起始复合物转录因子中的异常电荷配置。
Unusual charge configurations in transcription factors of the basic RNA polymerase II initiation complex.
作者信息
Karlin S
机构信息
Department of Mathematics, Stanford University, CA 94305.
出版信息
Proc Natl Acad Sci U S A. 1993 Jun 15;90(12):5593-7. doi: 10.1073/pnas.90.12.5593.
Abstract
A systematic analysis of the primary sequences of the polymerase II initiation complex has revealed unusual charge features in the TFII family proteins. In particular, the proteins TFIIA alpha, TFIIE alpha, and TFIIF carry multiple charge clusters and hyper charge runs, sequence features occurring in < 4% of all (available) eukaryotic proteins. Possible implications for these charge structures are discussed in relation to the assembly and function of the polymerase II transcriptional complex.
摘要
对聚合酶II起始复合物一级序列的系统分析揭示了TFII家族蛋白中不同寻常的电荷特征。特别是，TFIIAα、TFIIEα和TFIIF蛋白带有多个电荷簇和超电荷序列，这些序列特征在所有（可获得的）真核蛋白中出现的比例不到4%。文中讨论了这些电荷结构与聚合酶II转录复合物的组装和功能之间可能存在的关联。
相似文献
1
Unusual charge configurations in transcription factors of the basic RNA polymerase II initiation complex.基本RNA聚合酶II起始复合物转录因子中的异常电荷配置。
Proc Natl Acad Sci U S A. 1993 Jun 15;90(12):5593-7. doi: 10.1073/pnas.90.12.5593.
2
Dissection of transcription factor TFIIF functional domains required for initiation and elongation.转录起始和延伸所需转录因子TFIIF功能结构域的剖析。
Proc Natl Acad Sci U S A. 1995 Jun 20;92(13):6042-6. doi: 10.1073/pnas.92.13.6042.
3
TFIIF-TAF-RNA polymerase II connection.TFIIF-TAF-核糖核酸聚合酶II连接
Genes Dev. 1994 Dec 1;8(23):2868-78. doi: 10.1101/gad.8.23.2868.
4
Conserved sequence motifs in the small subunit of human general transcription factor TFIIE.人类通用转录因子TFIIE小亚基中的保守序列基序。
Nature. 1991 Dec 5;354(6352):401-4. doi: 10.1038/354401a0.
5
Structural and functional interactions of transcription factor (TF) IIA with TFIIE and TFIIF in transcription initiation by RNA polymerase II.转录因子IIA与TFIIE和TFIIF在RNA聚合酶II转录起始过程中的结构与功能相互作用。
J Biol Chem. 2001 Oct 19;276(42):38652-7. doi: 10.1074/jbc.M106422200. Epub 2001 Aug 16.
6
A mammalian SRB protein associated with an RNA polymerase II holoenzyme.一种与RNA聚合酶II全酶相关的哺乳动物SRB蛋白。
Nature. 1996 Mar 7;380(6569):82-5. doi: 10.1038/380082a0.
7
A transcription reinitiation intermediate that is stabilized by activator.一种由激活剂稳定的转录重新起始中间体。
Nature. 2000 Nov 9;408(6809):225-9. doi: 10.1038/35041603.
8
Structural motifs and potential sigma homologies in the large subunit of human general transcription factor TFIIE.人类通用转录因子TFIIE大亚基中的结构基序和潜在的sigma同源性
Nature. 1991 Dec 5;354(6352):398-401. doi: 10.1038/354398a0.
9
Evidence for a mediator cycle at the initiation of transcription.转录起始时介质循环的证据。
Proc Natl Acad Sci U S A. 1997 Jun 10;94(12):6075-8. doi: 10.1073/pnas.94.12.6075.
10
Prediction of the general transcription factors associated with RNA polymerase II in Plasmodium falciparum: conserved features and differences relative to other eukaryotes.恶性疟原虫中与RNA聚合酶II相关的通用转录因子的预测：相对于其他真核生物的保守特征和差异
BMC Genomics. 2005 Jul 23;6:100. doi: 10.1186/1471-2164-6-100.
引用本文的文献
1
The Delta subunit of RNA polymerase is required for virulence of Streptococcus agalactiae.无乳链球菌的毒力需要RNA聚合酶的δ亚基。
Infect Immun. 2003 Jul;71(7):4011-7. doi: 10.1128/IAI.71.7.4011-4017.2003.
2
3
Data transferability from model organisms to human beings: insights from the functional genomics of the flightless region of Drosophila.从模式生物到人类的数据可转移性：来自果蝇无翅区域功能基因组学的见解。
Proc Natl Acad Sci U S A. 1998 Mar 31;95(7):3731-6. doi: 10.1073/pnas.95.7.3731.
4
Genetic analysis of the large subunit of yeast transcription factor IIE reveals two regions with distinct functions.酵母转录因子IIE大亚基的遗传分析揭示了两个具有不同功能的区域。
Mol Cell Biol. 1997 Sep;17(9):5288-98. doi: 10.1128/MCB.17.9.5288.
5
Identification of RTF1, a novel gene important for TATA site selection by TATA box-binding protein in Saccharomyces cerevisiae.酿酒酵母中RTF1的鉴定，RTF1是一种对TATA框结合蛋白选择TATA位点很重要的新基因。
Mol Cell Biol. 1997 Aug;17(8):4490-500. doi: 10.1128/MCB.17.8.4490.
6
Copurification of casein kinase II with transcription factor ATF/E4TF3.酪蛋白激酶II与转录因子ATF/E4TF3的共纯化
Nucleic Acids Res. 1996 Mar 1;24(5):876-84. doi: 10.1093/nar/24.5.876.
7
Paf1p, an RNA polymerase II-associated factor in Saccharomyces cerevisiae, may have both positive and negative roles in transcription.Paf1p是酿酒酵母中一种与RNA聚合酶II相关的因子，在转录过程中可能兼具正向和负向作用。
Mol Cell Biol. 1996 Feb;16(2):669-76. doi: 10.1128/MCB.16.2.669.
8
Involvement of transcription factor YB-1 in human T-cell lymphotropic virus type I basal gene expression.转录因子YB-1参与I型人嗜T细胞病毒基础基因表达。
J Virol. 1994 Jan;68(1):561-5. doi: 10.1128/JVI.68.1.561-565.1994.
9
Cloning and characterization of an evolutionarily divergent DNA-binding subunit of mammalian TFIIIC.哺乳动物TFIIIC进化上不同的DNA结合亚基的克隆与特性分析
Mol Cell Biol. 1994 May;14(5):3053-64. doi: 10.1128/mcb.14.5.3053-3064.1994.
10
A mutation in the C31 subunit of Saccharomyces cerevisiae RNA polymerase III affects transcription initiation.酿酒酵母RNA聚合酶III的C31亚基中的突变影响转录起始。
EMBO J. 1995 Jan 16;14(2):351-9. doi: 10.1002/j.1460-2075.1995.tb07009.x.
本文引用的文献
1
A short amino acid sequence able to specify nuclear location.一段能够指定核定位的短氨基酸序列。
Cell. 1984 Dec;39(3 Pt 2):499-509. doi: 10.1016/0092-8674(84)90457-4.
2
Net N-C charge imbalance may be important for signal sequence function in bacteria.净N-C电荷失衡可能对细菌中的信号序列功能很重要。
J Mol Biol. 1986 Nov 20;192(2):287-90. doi: 10.1016/0022-2836(86)90365-7.
3
Anionic regions in nuclear proteins.核蛋白中的阴离子区域。
J Cell Biol. 1987 Oct;105(4):1479-82. doi: 10.1083/jcb.105.4.1479.
4
Deletion analysis of GAL4 defines two transcriptional activating segments.对GAL4的缺失分析确定了两个转录激活区段。
Cell. 1987 Mar 13;48(5):847-53. doi: 10.1016/0092-8674(87)90081-x.
5
Association of charge clusters with functional domains of cellular transcription factors.电荷簇与细胞转录因子功能域的关联
Proc Natl Acad Sci U S A. 1989 Aug;86(15):5698-702. doi: 10.1073/pnas.86.15.5698.
6
Investigating protein-protein interaction surfaces using a reduced stereochemical and electrostatic model.
J Mol Biol. 1989 Mar 20;206(2):381-95. doi: 10.1016/0022-2836(89)90487-7.
7
Highly conserved core domain and unique N terminus with presumptive regulatory motifs in a human TATA factor (TFIID).人类TATA因子（TFIID）中高度保守的核心结构域以及具有假定调控基序的独特N端。
Nature. 1990 Jul 26;346(6282):387-90. doi: 10.1038/346387a0.
8
Charge configurations in oncogene products and transforming proteins.
Oncogene. 1990 Jan;5(1):85-95.
9
Identification of significant sequence patterns in proteins.
Methods Enzymol. 1990;183:388-402. doi: 10.1016/0076-6879(90)83026-6.
10
Very long charge runs in systemic lupus erythematosus-associated autoantigens.系统性红斑狼疮相关自身抗原中存在极长的电荷序列。
Proc Natl Acad Sci U S A. 1991 Feb 15;88(4):1536-40. doi: 10.1073/pnas.88.4.1536.
Zotero 插件