Chaĭka V O, Havrysh L I, Khil'ko T D, Ostapchenko L I
Ukr Biokhim Zh (1999). 2005 Mar-Apr;77(2):118-22.
The goal of the work was to evaluate plasma membrane phospholipid composition in rat gastric parietal cells under the histamine H3 receptor activation. The content of cyclic nucleotides was also studied. It was shown that H3-receptor agonist (R)-alpha-methylhistamine increases the phosphatidylcholine (PC) and phosphatidylethanolamine (PE) level and decreases the phoshatidylinositole level in plasma membrane of rat gastric parietal cells and leads to attenuation of the cGMP production and enhancement of the cAMP production under the experimental stress--the induced stomach ulcer formation. The histamine H3-receptor antagonist, thioperamide, insignificantly increases the PC and PE level and increases the phoshatidylinositole level in the plasma membrane of rat gastric parietal cells and leads to cAMP production attenuation, and cGMP contents decreases in the above-stated cells. Thus it was shown that histamine H3 receptor activation causes different effects on polyphosphoinositide and adenylatcyclase cascades in parietal cells under stomach ulcer conditions.
这项工作的目的是评估组胺H3受体激活情况下大鼠胃壁细胞的质膜磷脂组成。同时也研究了环核苷酸的含量。结果显示，H3受体激动剂（R）-α-甲基组胺可增加大鼠胃壁细胞质膜中磷脂酰胆碱（PC）和磷脂酰乙醇胺（PE）的水平，并降低磷脂酰肌醇水平，且在实验应激——诱导胃溃疡形成的情况下，会导致cGMP生成减弱以及cAMP生成增强。组胺H3受体拮抗剂硫代哌啶可使大鼠胃壁细胞质膜中PC和PE水平略有增加，并使磷脂酰肌醇水平升高，导致cAMP生成减弱，且上述细胞中的cGMP含量降低。因此表明，在胃溃疡条件下，组胺H3受体激活对壁细胞中的多磷酸肌醇和腺苷酸环化酶级联反应会产生不同影响。
