Bures J, Kopácová M, Vorísek V, Bukac J, Neumann D, Rejchrt S, Pozler O, Douda T, Zivný P, Palicka V
Katedra interních oborů LF UK, Hradec Králové.
Cas Lek Cesk. 2005;144 Suppl 3:18-22.
13C-octanoic acid breath test (13C-OABT) is a simple, safe and non-invasive technique for measuring gastric emptying. However, the method has not been standardized yet. Aim of the study was to work up, introduce and evaluate our own method of the 13C-OABT for adults.
Ten healthy volunteers entered the study (5 men, 5 women, mean age 32 years, 50 % Helicobacter pylori positive). Standard test meals (with 100 mg 13C-sodium octanoate) were used three times within 3 weeks. The same solid meal (1,178 kJ) for Tests 1 and 2 contained scrambled egg (+ 3 g oil), white bread (40 g), butter (10 g) and distilled water (200 ml). Semi-solid meal (1,020 kJ) for Test 3 contained milk pudding (200 g) and distilled water (200 ml). Duplicate breath samples were obtained before and every 15 minutes after eating the test meal during 255 minutes. Altogether 1,080 breath samples were analysed twice (isotope ratio mass spectrometry, AP2003 Analytical Precision, UK). To assess the half-life of elimination (t1/2 E), we modelled the process of elimination with the incomplete gamma-function, which has a convenient form for the empiric plotting of breath test data. Mean t1/2E was 136+/-10 minutes (Test 1), 134+/-14 (Test 2) and 123+/-16 minutes (Test 3). Clinical reproducibility of 13C-OABT in particular persons was 98.2% (18 breath samples series), 90.8 % (15 samples) and 87.1% (9 breath samples series). There was a significant correlation between Test 1 and Test 2 results (r=0.887, p<0.0001). Mean difference of duplicate breath sample analysis was 1.460 % (in 540 pairs), mean baseline one-day analysis difference was 0.0982 (99.9274% accuracy). In healthy volunteers, normal range of t1/2E is 110-160 minutes for solids and 91-155 minutes for semisolid test meal. Using our own computed mean time of intermediate metabolism of 13C-octanoic acid (76.5+/-7.5 minutes), gastric emptying half-time is 33.5-83.5 minutes for solids and 14.5-78.5 minutes for semisolid test meal in healthy volunteers.
The 13C-OABT is accurate non-invasive method for gastric emptying measurement.
13C-辛酸呼气试验（13C-OABT）是一种用于测量胃排空的简单、安全且无创的技术。然而，该方法尚未标准化。本研究的目的是开发、引入并评估我们自己的成人13C-OABT方法。
10名健康志愿者参与了本研究（5名男性，5名女性，平均年龄32岁，50%幽门螺杆菌阳性）。在3周内使用标准测试餐（含100毫克13C-辛酸钠）3次。测试1和测试2使用相同的固体餐（1178千焦），包含炒鸡蛋（+3克油）、白面包（40克）、黄油（10克）和蒸馏水（200毫升）。测试3的半固体餐（1020千焦）包含牛奶布丁（200克）和蒸馏水（200毫升）。在进食测试餐后的255分钟内，于餐前及餐后每隔15分钟采集重复的呼气样本。共对1080份呼气样本进行了两次分析（采用同位素比率质谱法，英国AP2003分析精度仪器）。为评估消除半衰期（t1/2E），我们用不完全伽马函数对消除过程进行建模，该函数对于呼气测试数据的经验性绘图具有便利的形式。平均t1/2E在测试1中为136±10分钟，测试2中为134±14分钟，测试3中为123±16分钟。13C-OABT在特定个体中的临床可重复性在18份呼气样本系列中为98.2%，15份样本中为90.8%，9份呼气样本系列中为87.1%。测试1和测试2结果之间存在显著相关性（r = 0.887，p < 0.0001）。重复呼气样本分析的平均差异为1.460%（在540对样本中），基线一日分析的平均差异为0.0982（准确性为99.9274%）。在健康志愿者中，固体餐的t1/2E正常范围为110 - 160分钟，半固体测试餐为91 - 155分钟。利用我们自己计算的13C-辛酸中间代谢平均时间（76.5±7.5分钟），健康志愿者中固体餐的胃排空半衰期为33.5 - 83.5分钟，半固体测试餐为14.5 - 78.5分钟。
13C-OABT是一种准确的胃排空测量无创方法。
