Rathsam Catherine, Eaton Ruth E, Simpson Christine L, Browne Gina V, Valova Valentina A, Harty Derek W S, Jacques Nicholas A
Institute of Dental Research, Westmead Millennium Institute and Westmead Centre for Oral Health, Westmead, New South Wales, Australia.
J Proteome Res. 2005 Nov-Dec;4(6):2161-73. doi: 10.1021/pr0502471.
Compared with traditional two-dimensional (2D) proteome analysis of Streptococcus mutans grown as a biofilm from a planktonic culture at steady state (Rathsam et al., Microbiol. 2005, 151, 1823-1837), the use of 2D fluorescence difference gel electrophoresis (DIGE) led to a 3-fold increase in the number of identified protein spots that were significantly altered in their level of expression (P < 0.050). Of the 73 identified proteins, only nine were up-regulated in biofilm grown cells. The results supported the previously surmised hypothesis that general metabolic functions were down-regulated in response to a reduction in growth rate in mature S. mutans biofilms. Up-regulation of competence proteins without any concomitant increase in stress-responsive proteins was confirmed, while the levels of glucosyltransferase C (GtfC), involved in glucan formation, O-acetylserine sulfhyrylase (cysteine synthetase A; CsyK), implicated in the formation of [Fe-S] clusters, and a hypothetical protein encoded by the open reading frame, SMu0188, were also up-regulated.
与对变形链球菌在稳态下从浮游培养物形成的生物膜进行的传统二维（2D）蛋白质组分析相比（Rathsam等人，《微生物学》，2005年，第151卷，第1823 - 1837页），使用二维荧光差异凝胶电泳（DIGE）使鉴定出的表达水平有显著变化（P < 0.050）的蛋白质斑点数量增加了3倍。在鉴定出的73种蛋白质中，只有9种在生物膜生长的细胞中上调。结果支持了先前推测的假设，即成熟变形链球菌生物膜中生长速率降低会导致一般代谢功能下调。证实了感受态蛋白上调而应激反应蛋白没有任何相应增加，同时参与葡聚糖形成的葡糖基转移酶C（GtfC）、与[Fe - S]簇形成有关的O - 乙酰丝氨酸巯基酶（半胱氨酸合成酶A；CsyK）以及由开放阅读框SMu0188编码的一种假定蛋白的水平也上调。
