Division of Microbiology and Molecular Genetics, School of Medicine, Loma Linda University, Loma Linda, CA 92350, USA.
Proteomics. 2012 Nov;12(22):3343-64. doi: 10.1002/pmic.201200211.
Filifactor alocis, a Gram-positive anaerobic rod, is now considered one of the marker organisms associated with periodontal disease. Although there was heterogeneity in its virulence potential, this bacterium was shown to have virulence properties that may enhance its ability to survive and persist in the periodontal pocket. To gain further insight into a possible mechanism(s) of pathogenesis, the proteome of F. alocis strains was evaluated. Proteins including several proteases, neutrophil-activating protein A and calcium-binding acid repeat protein, were identified in F. alocis. During the invasion of HeLa cells, there was increased expression of several of the genes encoding these proteins in the potentially more virulent F. alocis D-62D compared to F. alocis ATCC 35896, the type strain. A comparative protein in silico analysis of the proteome revealed more cell wall anchoring proteins in the F. alocis D-62D compared to F. alocis ATCC 35896. Their expression was enhanced by coinfection with Porphyromonas gingivalis. Taken together, the variation in the pathogenic potential of the F. alocis strains may be related to the differential expression of several putative virulence factors.
栖牙密螺旋体是一种革兰氏阳性厌氧菌，现被认为是与牙周病相关的标志性生物之一。尽管其毒力潜力存在异质性，但该细菌具有增强其在牙周袋中生存和持续存在能力的毒力特性。为了更深入地了解可能的发病机制，评估了栖牙密螺旋体菌株的蛋白质组。在栖牙密螺旋体中鉴定到包括几种蛋白酶、中性粒细胞激活蛋白 A 和钙结合酸重复蛋白在内的蛋白质。在 HeLa 细胞侵袭过程中，与更具毒力的 F. alocis D-62D 相比，F. alocis ATCC 35896（模式株）编码这些蛋白质的几个基因的表达增加。对蛋白质组的比较蛋白质计算机分析显示，F. alocis D-62D 中的细胞壁锚定蛋白多于 F. alocis ATCC 35896。它们的表达通过与牙龈卟啉单胞菌的共感染而增强。总之，栖牙密螺旋体菌株的致病潜力的差异可能与几种潜在毒力因子的差异表达有关。
