Wong Julian L, Wessel Gary M
Department of Molecular Biology, Cellular Biology, and Biochemistry, Box G-J4, Brown University, Providence, RI 02912, USA.
Dev Biol. 2005 Dec 15;288(2):317-33. doi: 10.1016/j.ydbio.2005.07.004. Epub 2005 Dec 5.
Sea urchin fertilization is marked by a massive conversion of molecular oxygen to hydrogen peroxide by a sea urchin dual oxidase, Udx1. This enzyme is essential for completing the physical block to polyspermy. Yet, its expression is maintained during development, as indicated by the presence of both Udx1 mRNA and Udx1 protein enriched at the surface of all non-mesenchymal blastomeres. When hydrogen peroxide synthesis by Udx1 is inhibited, either pharmacologically or by specific antibody injection, cleavage is delayed. Application of exogenous hydrogen peroxide, however, partially rescues a fraction of these defective embryos. We also report an unequal distribution of reactive oxygen species between sister blastomeres during early cleavage stages, suggesting a functional role for Udx1 in intracellular signaling.
海胆受精的标志是海胆双氧化酶Udx1将大量分子氧转化为过氧化氢。这种酶对于完成对多精入卵的物理阻断至关重要。然而，正如所有非间充质卵裂球表面富集的Udx1 mRNA和Udx1蛋白所表明的那样，其表达在发育过程中持续存在。当通过药理学方法或注射特异性抗体抑制Udx1合成过氧化氢时，卵裂会延迟。然而，施加外源性过氧化氢可部分挽救这些有缺陷胚胎中的一部分。我们还报告了在早期卵裂阶段姐妹卵裂球之间活性氧物种的分布不均，这表明Udx1在细胞内信号传导中具有功能作用。
