Department of Biology and Center for Developmental Biology, University of Washington, Seattle, WA 98195, USA.
Mech Dev. 2013 Apr-May;130(4-5):290-303. doi: 10.1016/j.mod.2012.12.003. Epub 2013 Jan 8.
Is focal adhesion kinase (FAK) needed for embryonic cleavage? We find that FAK is expressed during early cleavage divisions of sea urchin embryos as determined by polyclonal antibodies to the Lytechinus variegatus protein. FAK is absent in eggs and zygotes and then cycles in abundance during the first cleavages after fertilization. It is maximal at anaphase, similar to the destruction and synthesis of cyclin proteins. To investigate whether FAK is needed during early cleavage, we interfered with its function by microinjecting eggs with anti-FAK antibodies or with FAK antisense morpholino oligonucleotides. Both treatments led to regression of the cleavage furrow. FAK knockdown with antibodies or morpholino oligonucleotides also resulted in an over-accumulation of endocytic vesicles. Thus, FAK could be restricting endocytosis or increasing exocytosis in localized areas important for abscission. FAK appears to be necessary for successful cleavage. These results are the first to document a functional role for FAK during embryonic cleavage.
黏着斑激酶（FAK）是否对胚胎卵裂有作用？我们发现黏着斑激酶在海胆胚胎的早期卵裂过程中表达，通过多克隆抗体对 Lytechinus variegatus 蛋白进行检测。FAK 在卵和受精卵中不存在，然后在受精后的第一次卵裂中大量循环。它在后期达到最大值，类似于细胞周期蛋白蛋白的破坏和合成。为了研究 FAK 在早期卵裂中是否需要，我们通过向卵中注射抗 FAK 抗体或 FAK 反义形态发生素寡核苷酸来干扰其功能。这两种处理都导致了卵裂沟的退化。用抗体或形态发生素寡核苷酸进行 FAK 敲低也导致内吞小泡的过度积累。因此，FAK 可能在对于分裂至关重要的局部区域限制内吞作用或增加胞吐作用。FAK 似乎对成功的卵裂是必要的。这些结果首次证明了 FAK 在胚胎卵裂过程中的功能作用。
