菠萝蛋白酶在卵清蛋白诱导的过敏性气道疾病小鼠模型中发挥抗炎作用。
Bromelain exerts anti-inflammatory effects in an ovalbumin-induced murine model of allergic airway disease.
作者信息
Secor Eric R, Carson William F, Cloutier Michelle M, Guernsey Linda A, Schramm Craig M, Wu Carol A, Thrall Roger S
机构信息
Department of Immunology, University of Connecticut, School of Medicine, Farmington, CT, USA.
出版信息
Cell Immunol. 2005 Sep;237(1):68-75. doi: 10.1016/j.cellimm.2005.10.002. Epub 2005 Dec 6.
OBJECTIVE
Bromelain, a clinically used pineapple extract and natural product, has reported anti-inflammatory and immunomodulatory activities. The purpose of this study was to determine the effect of bromelain treatment in an ovalbumin (OVA)-induced murine model of allergic airway disease (AAD).
METHODS
To establish AAD, mice were sensitized with intraperitoneal (i.p.) OVA/alum and challenged with daily OVA aerosols. Mice were treated i.p. with either saline, 2 or 6 mg/kg bromelain, twice daily for four consecutive days. Bronchoalveolar lavage leukocytes and cytokines, lung histology, airway hyperresponsiveness, and lymphocyte populations via flow cytometry were compared between groups.
RESULTS
Bromelain treatment of AAD mice resulted in reduced total BAL leukocytes, eosinophils, CD4+ and CD8+ T lymphocytes, CD4+/CD8+ T cell ratio, and IL-13.
CONCLUSION
Bromelain attenuated development of AAD while altering CD4+ to CD8+ T lymphocyte populations. The reduction in AAD outcomes suggests that bromelain may have similar effects in the treatment of human asthma and hypersensitivity disorders.
目的
菠萝蛋白酶是一种临床应用的菠萝提取物和天然产物，已有报道称其具有抗炎和免疫调节活性。本研究的目的是确定菠萝蛋白酶治疗对卵清蛋白（OVA）诱导的小鼠过敏性气道疾病（AAD）模型的影响。
方法
为建立AAD模型，小鼠经腹腔注射（i.p.）OVA/明矾致敏，并用每日OVA气雾剂激发。小鼠连续四天每天两次腹腔注射生理盐水、2或6mg/kg菠萝蛋白酶。比较各组之间的支气管肺泡灌洗白细胞和细胞因子、肺组织学、气道高反应性以及通过流式细胞术检测的淋巴细胞群体。
结果
用菠萝蛋白酶治疗AAD小鼠可使支气管肺泡灌洗总白细胞、嗜酸性粒细胞、CD4+和CD8+T淋巴细胞、CD4+/CD8+T细胞比值以及IL-13减少。
结论
菠萝蛋白酶可减轻AAD的发展，同时改变CD4+至CD8+T淋巴细胞群体。AAD结果的改善表明菠萝蛋白酶在治疗人类哮喘和超敏反应性疾病中可能具有相似的作用。
Zotero 插件