Smuc Tina, Rupreht Ruth, Sinkovec Jasna, Adamski Jerzy, Rizner Tea Lanisnik
Institute of Biochemistry, Medical Faculty, University of Ljubljana, Vrazov trg 2, 1000 Ljubljana, Slovenia.
Mol Cell Endocrinol. 2006 Mar 27;248(1-2):114-7. doi: 10.1016/j.mce.2005.10.013. Epub 2005 Dec 6.
Estrogen-dependent endometrial cancer is related to unopposed and prolonged estrogen stimulation. We examined the expression of estrogen-metabolizing enzymes in correlation with the ERalpha and ERbeta estrogen receptors in human endometrial Ishikawa adenocarcinoma cells and in endometrial cancer specimens and adjacent normal endometrium from the same patients. Real-time PCR analysis revealed that both estrogen receptors and selected estrogen-metabolizing enzymes were expressed in the Ishikawa cells and in endometrial tissue. We detected higher expression of ERalpha than ERbeta, higher expression of sulfatase than sulfotransferase and low expression of aromatase in the Ishikawa cells and the tissue, as well as higher levels of type 2 17beta-hydroxysteroid dehydrogenase (17beta-HSD) in normal and diseased tissue than in the Ishikawa cells. When we compared the expression in endometrial cancer samples and in the adjacent normal endometrium, ERalpha and ERbeta, sulfatase and sulfotransferase were seen to be downregulated in the majority of the cancerous tissue specimens.
雌激素依赖型子宫内膜癌与无对抗的长期雌激素刺激有关。我们检测了雌激素代谢酶的表达，并将其与雌激素受体ERα和ERβ在人子宫内膜Ishikawa腺癌细胞、来自同一患者的子宫内膜癌标本及相邻正常子宫内膜中的表达进行关联分析。实时PCR分析显示，雌激素受体和选定的雌激素代谢酶在Ishikawa细胞和子宫内膜组织中均有表达。我们在Ishikawa细胞和组织中检测到ERα的表达高于ERβ，硫酸酯酶的表达高于磺基转移酶，芳香化酶的表达较低，并且在正常和病变组织中2型17β-羟基类固醇脱氢酶（17β-HSD）的水平高于Ishikawa细胞。当我们比较子宫内膜癌样本和相邻正常子宫内膜中的表达时，发现大多数癌组织标本中ERα和ERβ、硫酸酯酶和磺基转移酶的表达均下调。
