Shono Takeshi, Shima Yuichi, Kondo Tsuyoshi, Suita Sachiyo
Department of Pediatric Surgery, Reproductive and Developmental Medicine, Graduate School of Medical Sciences, Kyushu University, 812-8582, Fukuoka, Japan.
J Pediatr Surg. 2005 Dec;40(12):1861-4. doi: 10.1016/j.jpedsurg.2005.08.027.
Phthalate esters have been shown to cause undescended testes (UT) in rats. It has been proposed that Leydig insulin-like factor 3 (INSL3) may play an important role in testicular descent. The aim of this study was to investigate the effect of mono-n-butyl phthalate (MBP) on both the INSL3 gene expression and the transabdominal testicular descent in rats.
On the 19th gestational day, the male fetuses that had been exposed to MBP on the 15th to 18th gestational days were dissected and the degree of testicular ascent was examined. Next, quantitative reverse transcriptase polymerase chain reaction was performed to analyze the testicular INSL3 gene expression. At 60 days of age, the testicular position was recorded in the rest of MBP-treated male offspring.
On the 19th gestational day, INSL3 messenger RNA expression was significantly decreased in the MBP-treated testes, and the associated degree of testicular ascent was significantly higher than in the controls. At 60 days of age, 12 (54.5%) of 22 rats had either unilateral or bilateral UT.
Prenatal MBP may inhibit the INSL3 gene expression associated with the transabdominal testicular ascent in fetal rats, thereby causing UT in postnatal offspring.
邻苯二甲酸酯已被证明可导致大鼠隐睾。有人提出，睾丸间质细胞胰岛素样因子3（INSL3）可能在睾丸下降过程中起重要作用。本研究的目的是探讨邻苯二甲酸单丁酯（MBP）对大鼠INSL3基因表达和经腹睾丸下降的影响。
在妊娠第19天，解剖在妊娠第15至18天暴露于MBP的雄性胎儿，并检查睾丸上升程度。接下来，进行定量逆转录聚合酶链反应以分析睾丸INSL3基因表达。在60日龄时，记录其余MBP处理的雄性后代的睾丸位置。
在妊娠第19天，MBP处理的睾丸中INSL3信使核糖核酸表达显著降低，且相关的睾丸上升程度显著高于对照组。在60日龄时，22只大鼠中有12只（54.5%）出现单侧或双侧隐睾。
产前MBP可能抑制与胎鼠经腹睾丸上升相关的INSL3基因表达，从而导致出生后后代隐睾。
