Department of Pediatric Surgery, Saga Medical Center Koseikan, 400, Nakahara, Kasemachi, Saga, 840-8571, Japan,
Environ Sci Pollut Res Int. 2014 Feb;21(4):3187-90. doi: 10.1007/s11356-013-2332-3. Epub 2013 Dec 6.
Phthalates are widely used as plasticizer in various consumer domestic products and are known to disturb the male reproductive function in rodents. This study investigated the involvement of oxidative stress and the atrophy of the testes in pubertal rats exposed to mono-n-butyl phthalate (MBP). Four-week-old pubertal male rats were separated into three groups. In group I, 21 rats were fed rat chow containing 2 % MBP for 3 days. In group II, 21 rats were fed rat chow containing 2 % MBP for 3 days and antioxidant vitamins C (250 mg/kg/day) and E (50 mg/kg/day) were injected daily. In group III, 21 rats were fed standard rat chow and used as controls. After 3 days, each testis was weighed and the germ cell development was evaluated using the Johnsen score. The urinary 8-hydroxy-2'-deoxyguanosine (8-OHdG) levels were measured as a biological marker of oxidative DNA damage. The mean testis weight was significantly lower for group I than groups II or III (p < 0.05). The mean Johnsen score was significantly lower for group I than for groups II or III (p < 0.05). Urinary 8-OHdG concentrations were higher in group I than in groups II or III. Short-time exposure to MBP may therefore induce oxidative DNA damage in rat testes, while antioxidant vitamins administered during exposure may protect against this stress.
邻苯二甲酸酯作为一种增塑剂广泛应用于各种消费类家用产品中，已知其会扰乱啮齿动物的雄性生殖功能。本研究调查了青春期大鼠暴露于邻苯二甲酸单丁酯（MBP）中，氧化应激和睾丸萎缩的情况。将 4 周龄的青春期雄性大鼠分为三组。在第 I 组中，21 只大鼠喂食含 2%MBP 的大鼠饲料 3 天。在第 II 组中，21 只大鼠喂食含 2%MBP 的大鼠饲料 3 天，同时每天注射抗氧化维生素 C（250mg/kg/天）和维生素 E（50mg/kg/天）。第 III 组中，21 只大鼠喂食标准大鼠饲料作为对照。3 天后，称取每个睾丸的重量，并使用约翰森评分评估生精细胞的发育情况。将尿 8-羟基-2'-脱氧鸟苷（8-OHdG）水平作为氧化 DNA 损伤的生物标志物进行测量。第 I 组的平均睾丸重量明显低于第 II 组或第 III 组（p<0.05）。第 I 组的平均约翰森评分明显低于第 II 组或第 III 组（p<0.05）。第 I 组的尿 8-OHdG 浓度高于第 II 组或第 III 组。因此，短时间暴露于 MBP 可能会导致大鼠睾丸发生氧化 DNA 损伤，而在暴露期间给予抗氧化维生素可能会对此应激起到保护作用。
