佛波酯诱导人红白血病细胞中血栓素合酶和前列腺素内过氧化物合酶的信使核糖核酸表达

Induction of thromboxane synthase and prostaglandin endoperoxide synthase mRNAs in human erythroleukemia cells by phorbol ester.

作者信息

Ihara H, Yokoyama C, Miyata A, Kosaka T, Nüsing R, Ullrich V, Tanabe T

机构信息

Department of Pharmacology, National Cardiovascular Center Research Institute, Osaka, Japan.

出版信息

FEBS Lett. 1992 Jul 20;306(2-3):161-4. doi: 10.1016/0014-5793(92)80990-x.

DOI:10.1016/0014-5793(92)80990-x

PMID:1633871

Abstract

The effects of 12-O-tetradecanoyl-phorbol-13-acetate (TPA) on the mRNA levels of two enzymes, thromboxane synthase (TXS) and prostaglandin endoperoxide synthase (PES), responsible for the synthesis of thromboxane A2 from arachidonic acid, were studied in human erythroleukemia (HEL) cells by RNA blot analysis. TPA induced both TXS and PES mRNAs in HEL cells in a dose-dependent manner at 36 h. The half-maximal and maximal effects for the induction of both mRNAs were at approximately 3 x 10(-9) M and at 10(-8) M, respectively. TXS and PES mRNA levels increased in a time-dependent fashion by TPA, and reached to 7- and 3.5-fold of the control, respectively after 48 h of TPA treatment. These results suggest that expression of TXS and PES genes in HEL cells were simultaneously stimulated by TPA.

摘要

通过RNA印迹分析，研究了12 - O - 十四烷酰佛波醇 - 13 - 乙酸酯（TPA）对人红白血病（HEL）细胞中两种酶——血栓素合酶（TXS）和前列腺素内过氧化物合酶（PES）mRNA水平的影响，这两种酶负责从花生四烯酸合成血栓素A2。TPA在36小时时以剂量依赖的方式诱导HEL细胞中的TXS和PES mRNA。两种mRNA诱导的半最大效应和最大效应分别约为3×10⁻⁹ M和10⁻⁸ M。TPA使TXS和PES mRNA水平随时间增加，在TPA处理48小时后分别达到对照的7倍和3.5倍。这些结果表明，TPA同时刺激了HEL细胞中TXS和PES基因的表达。