Research Group Functional Genomics, Research Institute for the Biology of Farm Animals (FBN), 18196 Dummerstorf, Germany.
Biol Reprod. 2006 Mar;74(3):577-84. doi: 10.1095/biolreprod.105.045096. Epub 2005 Dec 7.
A 3204-bp full-length cDNA of bovine NALP9 was cloned and its genomic organization was analyzed. The 2988-bp open reading frame covers 9 exons and encodes a deduced protein of 996 amino acids containing Pyrin, Nacht and leucine-rich repeat domains like the human NALP gene family members. Mapping with the WGRH5000 panel and fluorescence in situ hybridization assigned NALP9 in close vicinity to BM2078 (LOD score 25.71; distance 0.0 cR5000) on bovine chromosome 18, BTA18q25-q26, within a previously identified QTL region for reproductive traits flanked by the bovine marker BM2078 and TGLA227. BAC contig analysis revealed that NALP9, NALP8, and NALP5 map in this QTL region. Temporospatial expression of these members of the NALP gene family was monitored. Among the adult tissues examined, transcripts of NALP8 and NALP9 were detected exclusively in testis and ovary, whereas transcripts of the NALP5 gene are limited to the ovary. The transcripts of NALP9, NALP8, and NALP5 were detected in oocytes before and after in vitro maturation and with a gradual decline from 2-cell to 8-cell stage, suggesting no reactivation at the time of bovine maternal to embryonic transition. Assignment to a QTL region for reproductive traits and preferential expression of NALP9, NALP8, and NALP5 in oocyte, germinal lineage, and gonad cells may suggest their functional relevance to reproduction and possible contribution to phenotypic variation.
牛 NALP9 的全长 cDNA 被克隆，并分析了其基因组结构。该基因包含 9 个外显子，其开放阅读框长 2988bp，编码一个由 996 个氨基酸组成的蛋白，含有与人类 NALP 基因家族成员相似的 Pyrin、Nacht 和亮氨酸丰富重复结构域。通过与 WGRH5000 面板和荧光原位杂交定位，NALP9 位于牛 18 号染色体 BM2078 附近（LOD 得分 25.71；距离 0.0 cR5000），BTA18q25-q26，位于先前确定的生殖性状 QTL 区域内，该区域由牛标记物 BM2078 和 TGLA227 侧翼。BAC 连续分析显示 NALP9、NALP8 和 NALP5 位于该 QTL 区域内。这些 NALP 基因家族成员的时空表达被监测。在所检查的成年组织中，NALP8 和 NALP9 的转录本仅在睾丸和卵巢中检测到，而 NALP5 基因的转录本仅局限于卵巢。在体外成熟前后的卵母细胞中检测到 NALP9、NALP8 和 NALP5 的转录本，并且从 2 细胞期到 8 细胞期逐渐下降，这表明在牛母体到胚胎转变时没有重新激活。NALP9、NALP8 和 NALP5 在卵母细胞、生殖系和性腺细胞中的 QTL 区域的分配以及优先表达可能表明它们在生殖中的功能相关性和对表型变异的可能贡献。
